Aligments for a candidate for ytfR in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for ytfR in Klebsiella michiganensis M5al

Align galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized)
to candidate BWI76_RS07240 BWI76_RS07240 D-ribose transporter ATP-binding protein

Query= ecocyc::YTFR-MONOMER
         (500 letters)



>FitnessBrowser__Koxy:BWI76_RS07240
          Length = 494

 Score =  389 bits (1000), Expect = e-112
 Identities = 199/469 (42%), Positives = 308/469 (65%), Gaps = 4/469 (0%)

Query: 10  LRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGENGAGKSTLIKALTGVYHADRGTIWLE 69
           L  EG+SKFFPGVKALDNV   +R G + AL+GENGAGKSTL+K L G+Y  D+G I ++
Sbjct: 6   LEAEGISKFFPGVKALDNVSLRVRPGTVHALMGENGAGKSTLMKCLIGIYRPDKGAIRVK 65

Query: 70  GQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVADNLFIGREPKRFGLLRRKEMEKRATEL 129
           G+ +  ++T  A + GI  ++QE+NL+P+M+VA+N+++GREP ++G +  +++ ++  +L
Sbjct: 66  GEPVQFQDTMDALRSGISMIHQELNLVPHMTVAENIWLGREPMKYGFVDHRQLARQTQDL 125

Query: 130 MASYGFSLDVREPLNRFSVAMQQIVAICRAIDLSAKVLILDEPTASLDTQEVELLFDLMR 189
           +      L     +   S+A QQ+V I +A+  +A ++I+DEPT++L   EV  LF ++R
Sbjct: 126 LDKLNIRLSADRLVGELSIASQQMVEIAKAVSWNADIVIMDEPTSALTESEVAHLFTIIR 185

Query: 190 QLRDRGVSLIFVTHFLDQVYQVSDRITVLRNGSFVGCRETCELPQIELVKMMLGRELDTH 249
            LR +G ++I+++H +D+++ ++D I+V R+G++VG ++T E  +  L+  M+GREL T 
Sbjct: 186 DLRQQGKAIIYISHKMDEIFAITDEISVFRDGTWVGSKQTTEFTRQSLITQMVGREL-TQ 244

Query: 250 ALQRAGRTLLSDKPVAAFKNYGKKGTIAPFDLEVRPGEIVGLAGLLGSGRTETAEVIFGI 309
              +    +   + V   +N  +KG     +  VR GEI+G+AGL+G+GR+E  E +FG+
Sbjct: 245 LFPKFNNAI--GEEVLTVRNLSRKGAFHDINFSVRRGEILGVAGLVGAGRSEVMESLFGM 302

Query: 310 KPADSGTALIKGKPQNLRSPHQASVLGIGFCPEDRKTDGIIAAASVRENI-ILALQAQRG 368
           + ADSG  LI G P N+ SP  A   G+    EDRK  G+    SV EN+ I+ +    G
Sbjct: 303 EKADSGEVLIDGMPVNIDSPSTAIEKGMALLTEDRKKSGLFLVLSVLENMSIVKMPEYIG 362

Query: 369 WLRPISRKEQQEIAERFIRQLGIRTPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDE 428
               +   +  E     IR+L I+TP+ +Q I  LSGGNQQKVL++RWLL +P+ LILDE
Sbjct: 363 KTGFVQHLKMAEDCMEQIRRLNIKTPTMDQIINNLSGGNQQKVLIARWLLAQPKILILDE 422

Query: 429 PTRGIDVGAHAEIIRLIETLCADGLALLVISSELEELVGYADRVIIMRD 477
           PTRGIDVGA AEI  LI  L   G+A++++SSEL E++G +DRV++M +
Sbjct: 423 PTRGIDVGAKAEIYHLISELANRGVAVIMVSSELPEILGMSDRVMVMHE 471



 Score = 99.0 bits (245), Expect = 3e-25
 Identities = 67/227 (29%), Positives = 121/227 (53%), Gaps = 13/227 (5%)

Query: 7   QEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGENGAGKSTLIKALTGVYHADRGTI 66
           +E+L    LS+      A  +++FS+RRGEI+ + G  GAG+S ++++L G+  AD G +
Sbjct: 255 EEVLTVRNLSR----KGAFHDINFSVRRGEILGVAGLVGAGRSEVMESLFGMEKADSGEV 310

Query: 67  WLEGQAISPKNTAHAQQLGIGTVYQE---VNLLPNMSVADNLFIGREPKRFGLLRRKEME 123
            ++G  ++  + + A + G+  + ++     L   +SV +N+ I + P+  G     +  
Sbjct: 311 LIDGMPVNIDSPSTAIEKGMALLTEDRKKSGLFLVLSVLENMSIVKMPEYIGKTGFVQHL 370

Query: 124 KRATELMASYGFSLDVREP-----LNRFSVAMQQIVAICRAIDLSAKVLILDEPTASLDT 178
           K A + M      L+++ P     +N  S   QQ V I R +    K+LILDEPT  +D 
Sbjct: 371 KMAEDCMEQIR-RLNIKTPTMDQIINNLSGGNQQKVLIARWLLAQPKILILDEPTRGIDV 429

Query: 179 QEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVLRNGSFVG 225
                ++ L+ +L +RGV++I V+  L ++  +SDR+ V+  G   G
Sbjct: 430 GAKAEIYHLISELANRGVAVIMVSSELPEILGMSDRVMVMHEGRITG 476


Lambda     K      H
   0.321    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 600
Number of extensions: 30
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 500
Length of database: 494
Length adjustment: 34
Effective length of query: 466
Effective length of database: 460
Effective search space:   214360
Effective search space used:   214360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources