Aligments for a candidate for gntT in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for gntT in Klebsiella michiganensis M5al

Align Low-affinity (212 (characterized)
to candidate BWI76_RS26235 BWI76_RS26235 gluconate transporter

Query= TCDB::P0AC96
         (446 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS26235 BWI76_RS26235 gluconate
           transporter
          Length = 446

 Score =  843 bits (2179), Expect = 0.0
 Identities = 431/446 (96%), Positives = 443/446 (99%)

Query: 1   MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGG 60
           M+TLTLVLTAVGSVLLLLFLVMKARMHAF+ALMVVS+GAGLFSGMPLDKIAATMEKGMGG
Sbjct: 1   MSTLTLVLTAVGSVLLLLFLVMKARMHAFVALMVVSIGAGLFSGMPLDKIAATMEKGMGG 60

Query: 61  TLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALPLFFEV 120
           TLGFLA+VVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAHYAIGLAGL+CALPLFFEV
Sbjct: 61  TLGFLAIVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAHYAIGLAGLICALPLFFEV 120

Query: 121 AIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNADFGWMIL 180
           AIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFL+PGPAPMLLASQM+ADFGWMIL
Sbjct: 121 AIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLLPGPAPMLLASQMHADFGWMIL 180

Query: 181 IGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV 240
           IGLCAAIPGMIIAGPLWGNFISRYVELH PDDISEPHLGEGKMPSFGFSL+LILLPLVLV
Sbjct: 181 IGLCAAIPGMIIAGPLWGNFISRYVELHTPDDISEPHLGEGKMPSFGFSLALILLPLVLV 240

Query: 241 GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHAL 300
           GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLA+RQGM KD+VMEICGHAL
Sbjct: 241 GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAVRQGMAKDRVMEICGHAL 300

Query: 301 QPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATV 360
           QPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATV
Sbjct: 301 QPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATV 360

Query: 361 ACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETL 420
           ACLTAVGLVMPVIEQL+YSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEA+TL
Sbjct: 361 ACLTAVGLVMPVIEQLHYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAQTL 420

Query: 421 KTWTMMETILGTVGAIVGMIAFQLLS 446
           KTWTMMETILGTVGAIVGMIAF LLS
Sbjct: 421 KTWTMMETILGTVGAIVGMIAFTLLS 446


Lambda     K      H
   0.328    0.142    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 831
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 446
Length of database: 446
Length adjustment: 32
Effective length of query: 414
Effective length of database: 414
Effective search space:   171396
Effective search space used:   171396
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources