Alignments for a candidate for nagF in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Klebsiella michiganensis M5al

Align N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA (characterized)
to candidate BWI76_RS10675 BWI76_RS10675 phosphoenolpyruvate--protein phosphotransferase

Query= TCDB::Q9HXN5
         (842 letters)



>FitnessBrowser__Koxy:BWI76_RS10675
          Length = 833

 Score =  333 bits (853), Expect = 3e-95
 Identities = 231/658 (35%), Positives = 336/658 (51%), Gaps = 37/658 (5%)

Query: 185 GLHARPAALLRKTAQGFSSQAE---LHFAGQVASVDSLVGIMGLGVAEQDEVEVICRGED 241
           G+HARPA+L+      FSS  E   +   G+  +  S + I+G    + D  E+I  GED
Sbjct: 14  GVHARPASLVETLCNTFSSSVEWLNIRTEGR-GNAKSALAIIGTNTLKGDRCELIVSGED 72

Query: 242 SEAALGALLAALASATAGAPKDAPRAIAPGEPARPAAVAGTLAG--------VCASPGLA 293
            E A  AL A +         DAP   A     +P   + +           VCA  G A
Sbjct: 73  EEQAFAALSAFIRDEFPHC--DAPLPTAEQMDVQPVPESLSRLNPTLFHALPVCA--GSA 128

Query: 294 SGPLARLGAISLP---ADDGRHRPEEQHLALDQALQRVRDDVQGSLQQARLGGDENEAAI 350
            G L  L ++ L    A       E++  ALD+ L  +  +++  L    L  D   +AI
Sbjct: 129 GGRLTHLKSLDLRDLGALPAAGAIEQEQAALDKGLTLLVKNIEFRL----LDNDGTASAI 184

Query: 351 FSAHLALLEDPGLLDAADMLIDQGVGAAHAWHRAIQAQCEILQALGNLLLAERANDLRDL 410
             AH +L  D  L       +  G+  A A   + +  C    A GN  L ER  D+RD+
Sbjct: 185 LEAHRSLATDASLRQHLLDGLLSGLSCAEAVVASGEHFCAQFSASGNSYLQERVLDVRDV 244

Query: 411 EKRVLRVLLGDT---APLRVPAGAIVAAREITPSDLAPLVDAGAAGLCMAEGGATSHVAI 467
             ++L+ + G+    AP ++   AI  A E+TPS    L      GL +  GG TSH  I
Sbjct: 245 CFQLLQHIYGEARFPAPGKLTEEAICLADELTPSQFLELDKTLLKGLLLRSGGTTSHTVI 304

Query: 468 LARSKGLPCLVALGAGLLELEEGRQVVLDAGQGRLELSPD---ARRLEQVALQVAQREEQ 524
           LARS  +P LV +    L     R+V +D   G + ++P+   AR  +Q A   AQ    
Sbjct: 305 LARSFNIPTLVGVDMEALLPWVDRRVQIDGNAGLVVVNPNQAVARYYQQEAWVQAQI--- 361

Query: 525 RRRQQADAQREALTRDGRRIEIGANVASPREAAEAFANGADGVGLLRTEFLFLERRAAPD 584
           RR+QQA   +E  T DG R+E+ AN+A   EA  AF NGA  VGL RTE L+++R +AP 
Sbjct: 362 RRQQQAWLDKEGRTEDGIRLEVAANIAHSVEATAAFNNGAQSVGLFRTEMLYMDRPSAPS 421

Query: 585 EEEQRNAYQEVLDAMGQRKVIIRTIDVGGDKHLDYLPLPVEENPALGLRGIHLGQARPEL 644
           E E  N + + L+    R +IIRT+D+GGDK + YL +P E NP LG R + +      L
Sbjct: 422 ENELYNIFCQALEPANGRGIIIRTMDIGGDKPVAYLNIPAENNPFLGYRAVRIYAEYQAL 481

Query: 645 LDQQLRALLRVEPLERCRILLPMVSEVDELRAIRRRLGELATQLGIERLP-----ELGVM 699
              QLRA+LR       +I++PM+S ++E+  ++ +L +    L  E +P      LG+M
Sbjct: 482 FRTQLRAILRASAHGALKIMIPMISSMEEILWVKEQLADAKQSLRSEHIPFDEKIPLGIM 541

Query: 700 IEVPSAALLADQLAEHADFLSIGTNDLSQYALAMDRCHAGLADRIDALHPALLRLIAQTC 759
           +EVPS   + DQ  E  DF SIG+NDL+QY LA+DR +A +    ++L+PA LR +    
Sbjct: 542 LEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAV 601

Query: 760 AGAARHGRWVGVCGALASDPLATPVLVGLGVEELSVGPNLVGEIKTRVRQLDAAECRR 817
               R G+W+G+CG L +     P+LVGLG++ELS+    +   K R+ QLD+  CR+
Sbjct: 602 QAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPATKARLAQLDSRACRQ 659


Lambda     K      H
   0.319    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1415
Number of extensions: 52
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 842
Length of database: 833
Length adjustment: 42
Effective length of query: 800
Effective length of database: 791
Effective search space:   632800
Effective search space used:   632800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources