Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate BWI76_RS08450 BWI76_RS08450 fumarate hydratase
Query= BRENDA::P0AC33 (548 letters) >FitnessBrowser__Koxy:BWI76_RS08450 Length = 550 Score = 768 bits (1983), Expect = 0.0 Identities = 367/545 (67%), Positives = 441/545 (80%), Gaps = 1/545 (0%) Query: 3 NKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFML 62 +KPF +Q F D+TEY LLT++HV V+E +G+E++KVAPEALTLLA+QAF++ASF L Sbjct: 2 SKPFVWQELFVQSNDNTEYELLTNQHVKVTELDGEEVIKVAPEALTLLAQQAFYEASFFL 61 Query: 63 RPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVW 122 R H +Q+A IL DP+AS NDKYVALQ LRN++++AKGVLP CQDTGTA IV KGQ VW Sbjct: 62 RAGHLKQIASILHDPQASSNDKYVALQLLRNAEVSAKGVLPNCQDTGTATIVASKGQNVW 121 Query: 123 TGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLC 182 TGG D AL++G+Y T+ E+NLRYSQNAPLDMY EVNT TNLPAQID+ A G EY+FL Sbjct: 122 TGGNDAEALSQGIYTTFQENNLRYSQNAPLDMYTEVNTQTNLPAQIDISAAPGSEYRFLF 181 Query: 183 IAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNL 242 + KGGGSANK L+QETK++L P KL +L EKM+ LGTAACPPYHIAFV+GG SA+ L Sbjct: 182 VNKGGGSANKAALFQETKSILQPEKLTAFLTEKMKALGTAACPPYHIAFVVGGLSADQAL 241 Query: 243 KTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIR 302 K KLAS KYYD LPT GNE GQAFRD LE LL ++ G+GAQFGGKYFAHDIRVIR Sbjct: 242 KVAKLASTKYYDNLPTSGNELGQAFRDTSLEAALLKASREFGIGAQFGGKYFAHDIRVIR 301 Query: 303 LPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVD 362 LPRHG SCP+ M +SCSADRNIKAKIN+ GIW+EKLEHNPGK+IP+ R ++V++D Sbjct: 302 LPRHGGSCPIAMALSCSADRNIKAKINKHGIWLEKLEHNPGKFIPDSQRIENGAQSVQLD 361 Query: 363 LNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYA 422 LNRP++EIL ++S PV TRLSL+G I+V RDIAHAKLKER+DNGE +P+Y+++H +YYA Sbjct: 362 LNRPLREILREMSTLPVGTRLSLSGPIVVARDIAHAKLKERLDNGEPMPEYMRNHIVYYA 421 Query: 423 GPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYL 482 GPAKTP A GSLGPTT GRMD YVD QA GGS+IML+KGNRSQQVTDAC KHGGF L Sbjct: 422 GPAKTPNNLACGSLGPTTGGRMDGYVDAFQAAGGSLIMLSKGNRSQQVTDACHKHGGFNL 481 Query: 483 GSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQ-LT 541 GSIGG AA+LAQ +KSL C+EYPELGMEA+W +EV++ PAF+LVDDKGN+FF Q + Sbjct: 482 GSIGGAAALLAQQYVKSLRCLEYPELGMEAVWMMEVDNLPAFVLVDDKGNNFFSQFEHQH 541 Query: 542 QCTRC 546 +C C Sbjct: 542 RCATC 546 Lambda K H 0.317 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1025 Number of extensions: 28 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 548 Length of database: 550 Length adjustment: 36 Effective length of query: 512 Effective length of database: 514 Effective search space: 263168 Effective search space used: 263168 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory