Alignments for a candidate for fumD in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for fumD in Klebsiella michiganensis M5al

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate BWI76_RS16115 BWI76_RS16115 fumarate hydratase

Query= BRENDA::P14407
         (548 letters)



>FitnessBrowser__Koxy:BWI76_RS16115
          Length = 551

 Score =  981 bits (2537), Expect = 0.0
 Identities = 474/548 (86%), Positives = 510/548 (93%)

Query: 1   MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF 60
           MS KPF+YQ  FP+ KD TEYYLL+ ++VS+A+F+G+ ILKV+P+ALTLLAQQAFHDA+F
Sbjct: 1   MSTKPFVYQEAFPLAKDKTEYYLLSKEHVSIAEFEGQEILKVDPQALTLLAQQAFHDAAF 60

Query: 61  MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR 120
           MLR +HQKQVA IL DPEAS+NDKYVALQFLRNS+IAAKGVLPTCQDTGTAII+GKKGQR
Sbjct: 61  MLRVSHQKQVAQILLDPEASDNDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIMGKKGQR 120

Query: 121 VWTGGGDEETLSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF 180
           VWTGGGDE  L++GVYNTY EDNLRYSQNA LDMYKEVNTGTNLPAQIDLYA DG+EYKF
Sbjct: 121 VWTGGGDEAALARGVYNTYTEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYATDGEEYKF 180

Query: 181 LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET 240
           LC+AKGGGSANKTYLYQETKAL+TP KLKN+LVEKMRTLGTAACPPYHIAFVIGGTSAE+
Sbjct: 181 LCIAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAES 240

Query: 241 NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRV 300
            LKTVKLAS HYYDELPTEGNEHGQAFRD+QLEQELL EAQ LGLGAQFGGKYFAHDIRV
Sbjct: 241 TLKTVKLASTHYYDELPTEGNEHGQAFRDIQLEQELLAEAQNLGLGAQFGGKYFAHDIRV 300

Query: 301 IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVK 360
           IRLPRHGASCPVGMGVSCSADRNIKAKINR GIWIEKLE NPGQYIP+ LRQ GEG  V 
Sbjct: 301 IRLPRHGASCPVGMGVSCSADRNIKAKINRHGIWIEKLESNPGQYIPEHLRQQGEGRVVS 360

Query: 361 VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY 420
           ++LN+PM EILAQLS +PVSTRL+L GTIIV RDIAHAKLKELID G+ELPQY+KDHPIY
Sbjct: 361 INLNQPMSEILAQLSAHPVSTRLTLNGTIIVARDIAHAKLKELIDNGEELPQYVKDHPIY 420

Query: 421 YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF 480
           YAGPAKTP GY SGSLGPTTAGRMDSYVDLLQSHG SM+MLAKGNRSQQVTDACHKHGGF
Sbjct: 421 YAGPAKTPEGYASGSLGPTTAGRMDSYVDLLQSHGASMVMLAKGNRSQQVTDACHKHGGF 480

Query: 481 YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVN 540
           YLGSIGGPAAVLAQQSIK LECVAYPELGMEAIWKIEVE+FPAFILVDDKGNDFFQQI N
Sbjct: 481 YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVENFPAFILVDDKGNDFFQQIQN 540

Query: 541 KQCANCTK 548
           KQCA C++
Sbjct: 541 KQCAGCSQ 548


Lambda     K      H
   0.317    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1114
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 551
Length adjustment: 36
Effective length of query: 512
Effective length of database: 515
Effective search space:   263680
Effective search space used:   263680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources