GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gltJ in Klebsiella michiganensis M5al

Align Glutamate/aspartate import permease protein GltJ (characterized)
to candidate BWI76_RS08105 BWI76_RS08105 glutamate/aspartate ABC transporter permease GltJ

Query= SwissProt::P0AER3
         (246 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS08105 BWI76_RS08105
           glutamate/aspartate ABC transporter permease GltJ
          Length = 246

 Score =  456 bits (1172), Expect = e-133
 Identities = 221/246 (89%), Positives = 231/246 (93%)

Query: 1   MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRF 60
           MSIDWNWGIFLQQAPFGNTTYLGW+WSGFQVT+ LS+ AWIIAF VGS FGILRTVPNR 
Sbjct: 1   MSIDWNWGIFLQQAPFGNTTYLGWLWSGFQVTVTLSVTAWIIAFFVGSLFGILRTVPNRL 60

Query: 61  LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL 120
           LS +GT YVELFRNVPLIVQFFTWYLV+PELLPE IGMWFK+ELDPNIQFF+SSMLCLGL
Sbjct: 61  LSTIGTCYVELFRNVPLIVQFFTWYLVVPELLPENIGMWFKSELDPNIQFFVSSMLCLGL 120

Query: 121 FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV 180
           FTAARVCEQVR+ IQSLPRGQKNA LAMGLTLPQ YRYVLLPNAYRVIVPPMTSEMMNLV
Sbjct: 121 FTAARVCEQVRSGIQSLPRGQKNAGLAMGLTLPQTYRYVLLPNAYRVIVPPMTSEMMNLV 180

Query: 181 KNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP 240
           KNSAIASTIGL DMAAQAGKLLDYSAHAWESFTAITLAYV INA IML+M +VERKVRLP
Sbjct: 181 KNSAIASTIGLADMAAQAGKLLDYSAHAWESFTAITLAYVFINAVIMLIMYVVERKVRLP 240

Query: 241 GNMGGK 246
           GNMGGK
Sbjct: 241 GNMGGK 246


Lambda     K      H
   0.328    0.140    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 349
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 246
Length of database: 246
Length adjustment: 24
Effective length of query: 222
Effective length of database: 222
Effective search space:    49284
Effective search space used:    49284
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory