Alignments for a candidate for dhaK' in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for dhaK' in Klebsiella michiganensis M5al

Align triokinase (EC 2.7.1.28); glycerone kinase (EC 2.7.1.29); FAD-AMP lyase (cyclizing) (EC 4.6.1.15) (characterized)
to candidate BWI76_RS24595 BWI76_RS24595 dihydroxyacetone kinase subunit DhaK

Query= BRENDA::Q3LXA3
         (575 letters)



>FitnessBrowser__Koxy:BWI76_RS24595
          Length = 356

 Score =  226 bits (577), Expect = 9e-64
 Identities = 144/356 (40%), Positives = 194/356 (54%), Gaps = 26/356 (7%)

Query: 4   KKLVNSVAGCADDALAGLVACNPNLQLLQGHRVALRSDLDSLKGRVALLSGGGSGHEPAH 63
           KKL+N V     + LAGL   +P L+L Q      R+D   + G+VALLSGGGSGHEP H
Sbjct: 2   KKLINRVEDVLSEQLAGLAKAHPELRLHQDPVYVTRADAP-VAGKVALLSGGGSGHEPMH 60

Query: 64  AGFIGKGMLTGVIAGAVFTSPAVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLARE 123
            G+IG+GML+G   G +FTSP    +     A+   G  G LLI+KNYTGD LNF  A E
Sbjct: 61  CGYIGQGMLSGACPGEIFTSPTPDKMFEC--AMNIDGGEGVLLIIKNYTGDILNFETATE 118

Query: 124 QARAEGIPVEMVVIGDDSAF-TVLKKAGRRGLCGTVLIHKVAGALAEAGVGLEEIAKQVN 182
                G+ V  VV+ DD A    L  AGRRG+  TVLI K+ GA AE G  LE  A+   
Sbjct: 119 LLHESGVKVTTVVVDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLESCAELGR 178

Query: 183 VVAKAMGTLGVSLSSCSVPGS-KPTFELSADEVELGLGIHGEAGVRRIKMATADEIVKLM 241
            +     ++G++L +C+VP + +P+FEL  DE+E G+GIHGE G+ R +  + D  V  M
Sbjct: 179 KLNNLGHSIGIALGACTVPAAGQPSFELQDDEMEFGVGIHGEPGIDRRRFTSLDRTVDEM 238

Query: 242 LD-------------HMTNTTNA------SHVPVQPGSSVVMMVNNLGGLSFLELGIIAD 282
            D             H  N   A      +   +Q G  V+ +VNNLG     EL  + +
Sbjct: 239 FDTLLENGAYSRTLRHWDNVKGAWQEVAQTKQALQKGDRVIALVNNLGATPLSELYGVYN 298

Query: 283 ATVRSLEGRGVKIARALVGTFMSALEMPGISLTLLLVDEPLLKLIDA--ETTAAAW 336
              +  E  G+ I R L+G++ ++L+M G S+TLL VD+  L L DA   T A  W
Sbjct: 299 RLTQRCEETGIVIERNLIGSYCTSLDMVGFSITLLKVDDETLALWDAPVHTPALNW 354


Lambda     K      H
   0.315    0.130    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 462
Number of extensions: 16
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 575
Length of database: 356
Length adjustment: 33
Effective length of query: 542
Effective length of database: 323
Effective search space:   175066
Effective search space used:   175066
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources