Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate BWI76_RS09505 BWI76_RS09505 arginine transporter permease subunit ArtM
Query= TCDB::Q9HU29 (230 letters) >FitnessBrowser__Koxy:BWI76_RS09505 Length = 222 Score = 155 bits (393), Expect = 5e-43 Identities = 81/214 (37%), Positives = 139/214 (64%), Gaps = 3/214 (1%) Query: 7 ILKWMPKMLQGAALTLELLAIAVVAGLALALPLGIARASRHWYVRAVPYAYIFFFRGTPL 66 +L ++P++L+G +L L A ++V L L+L I + + + YI F GTPL Sbjct: 1 MLDYLPELLKGLHTSLTLTAASIVVALILSLIFTIILTLKTPVLVWIVRGYITLFTGTPL 60 Query: 67 LLQLFIVYYGLAQFEEVRK-SAFWPYLRDPYWCALLTMTLHTAAYIAEILRGAIHSVPVG 125 L+Q+F++YYG QF +++ W + +P+ CAL+ ++L++AAY ++ GAI ++P G Sbjct: 61 LVQIFLIYYGPGQFPSLQEYPVLWHLISEPWLCALIALSLNSAAYTTQLFHGAIRAIPDG 120 Query: 126 EVEAARALGMSRRQALWHIILPRAVRIGLPAYSNEVILMLKASAVVYTVTLFDIMGMART 185 + ++ ALGMS++ L I+LP A + L +YSNEV+L+ K++++ YT+TL ++MG ++ Sbjct: 121 QWQSCGALGMSKKDTL-AILLPYAFKRALSSYSNEVVLVFKSTSLAYTITLMEVMGHSQL 179 Query: 186 IIARTYESMLFFCLAGALYLVITIVLTRIFRLIE 219 + RTY+ M+F AG +YLV+ +LT + RLIE Sbjct: 180 LYGRTYDVMVFGA-AGIVYLVVNGLLTLLMRLIE 212 Lambda K H 0.332 0.142 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 146 Number of extensions: 11 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 230 Length of database: 222 Length adjustment: 22 Effective length of query: 208 Effective length of database: 200 Effective search space: 41600 Effective search space used: 41600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory