GapMind for catabolism of small carbon sources

 

Aligments for a candidate for hisQ in Klebsiella michiganensis M5al

Align Probable permease of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate BWI76_RS10280 BWI76_RS10280 polar amino acid ABC transporter permease

Query= TCDB::Q9HU30
         (231 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS10280 BWI76_RS10280 polar amino
           acid ABC transporter permease
          Length = 241

 Score =  118 bits (295), Expect = 1e-31
 Identities = 74/209 (35%), Positives = 113/209 (54%), Gaps = 11/209 (5%)

Query: 11  QLLAGTWMTLKLSLAAVCVGLLLGLLGAIAKTSKYAALRFLGGTYTTIVRGVPETLWVLM 70
           + + G WMTL ++L ++  G++LGL+ A+ + + + A + L   Y  + RG P    ++ 
Sbjct: 14  EFVQGAWMTLLITLCSLLCGVVLGLVLALLQEAPFRAGKGLAFFYLWLFRGTPVLFQIIF 73

Query: 71  IYFGTVSGLNALGDLFGKPDLALSPFAAGTLALGLCFGAYATEVFRGALLSIPRGHREAG 130
           +Y       N L   FG   L  S F    LAL L  GAY  E+ R  L ++  G R AG
Sbjct: 74  VY-------NVLPG-FG---LRFSAFTCAVLALSLNEGAYMAEILRSGLQAVKSGQRTAG 122

Query: 131 QALGLSPGRIFWRIVLPQIWRVALPGLGNLYLILLKDTALVSLITLDEIMRKAQVASNAT 190
            ALG++ G+I  +IVLPQ  R+ LP +GN  + +LK +ALVS+I + E++  A   ++A+
Sbjct: 123 MALGMTSGQIMRKIVLPQAARIVLPPMGNQMISMLKSSALVSVIAVQELLLVANQTASAS 182

Query: 191 KEPFTFYMTAAAIYLSLTVVIMVALHFLE 219
              F     A   YL LT + MV   +LE
Sbjct: 183 FRYFEALCAAGIYYLLLTSLFMVFQSWLE 211


Lambda     K      H
   0.327    0.143    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 127
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 231
Length of database: 241
Length adjustment: 23
Effective length of query: 208
Effective length of database: 218
Effective search space:    45344
Effective search space used:    45344
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory