GapMind for catabolism of small carbon sources

 

Finding step fadA for L-isoleucine catabolism in Klebsiella michiganensis M5al

5 candidates for fadA: 2-methylacetoacetyl-CoA thiolase

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi BWI76_RS20460 acetyl-CoA C-acyltransferase FadI 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 (characterized) 88% 100% 758.8 3-keto-acyl-CoA-thiolase 35% 229.2
hi BWI76_RS20460 acetyl-CoA C-acyltransferase FadI fadI: acetyl-CoA C-acyltransferase FadI (EC 2.3.1.16) (TIGR02446) 100% 854.3 3-keto-acyl-CoA-thiolase 35% 229.2
hi BWI76_RS01360 acetyl-CoA C-acyltransferase FadA 3-ketoacyl-CoA thiolase FadA; Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 (characterized) 93% 100% 716.5
hi BWI76_RS01360 acetyl-CoA C-acyltransferase FadA fadA: acetyl-CoA C-acyltransferase FadA (EC 2.3.1.16) (TIGR02445) 100% 788.5
hi BWI76_RS23445 acetyl-CoA acetyltransferase acetyl-CoA C-acetyltransferase (EC 2.3.1.16) (characterized) 66% 100% 534.6
hi BWI76_RS23445 acetyl-CoA acetyltransferase acetyl-CoA C-acyltransferase (EC 2.3.1.16) (TIGR01930) 100% 476.9
med BWI76_RS16135 acetyl-CoA acetyltransferase subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized) 68% 100% 543.5 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) 72% 569.3
med BWI76_RS16135 acetyl-CoA acetyltransferase acetyl-CoA C-acyltransferase (EC 2.3.1.16) (TIGR01930) 100% 446.1 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) 72% 569.3
med BWI76_RS20455 multifunctional fatty acid oxidation complex subunit alpha long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211); acetyl-CoA C-acyltransferase (EC 2.3.1.16) (characterized) 42% 93% 529.3 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 80% 1130.2

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.

Also see fitness data for the candidates

Definition of step fadA

Or cluster all characterized fadA proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory