Align 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 (characterized)
to candidate BWI76_RS20460 BWI76_RS20460 acetyl-CoA C-acyltransferase FadI
Query= SwissProt::P76503 (436 letters) >FitnessBrowser__Koxy:BWI76_RS20460 Length = 436 Score = 758 bits (1958), Expect = 0.0 Identities = 385/436 (88%), Positives = 413/436 (94%) Query: 1 MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE 60 M Q LPLVTRQGDRIAIVSGLRTPFARQATAFHG+PAVDLGK+VVGE+LARS IPA++IE Sbjct: 1 MSQALPLVTRQGDRIAIVSGLRTPFARQATAFHGVPAVDLGKLVVGEMLARSTIPADIIE 60 Query: 61 QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA 120 QLVFGQVVQMPEAPNIAREIVLGTGM+VHTDAYSVSRACATSFQAVANVAESLMAGTIRA Sbjct: 61 QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRA 120 Query: 121 GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST 180 GIAGGADSSSVLPIGVSKKLAR LVD NKART+ QRLKLFSRLR RDL+PVPPAVAEYST Sbjct: 121 GIAGGADSSSVLPIGVSKKLARTLVDANKARTLGQRLKLFSRLRFRDLLPVPPAVAEYST 180 Query: 181 GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE 240 GLRMGDTAEQMAKT+GITRE+QDALA RSHQ AA+AW +GKL EVMTA+ PP+++PL + Sbjct: 181 GLRMGDTAEQMAKTWGITRERQDALALRSHQLAAKAWEEGKLSAEVMTAYTPPFREPLEQ 240 Query: 241 DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY 300 DNNIR NS+LADY KLRPAFDR+HG+VTAANSTPLTDGAAAVILMTESRAKELGLVPLGY Sbjct: 241 DNNIRKNSTLADYQKLRPAFDRQHGSVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY 300 Query: 301 LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE 360 LRSYAFTAIDV QDMLLGPAWSTPLALERAGLT++DLTLIDMHEAFAAQTLAN+ + S+ Sbjct: 301 LRSYAFTAIDVRQDMLLGPAWSTPLALERAGLTLADLTLIDMHEAFAAQTLANLHCMASD 360 Query: 361 RFAREALGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT 420 RFARE LGR+ A GEVD+SKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT Sbjct: 361 RFAREVLGRSQAIGEVDESKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT 420 Query: 421 ACAAGGLGAAMVLEAE 436 ACAAGGLG+AMVLEAE Sbjct: 421 ACAAGGLGSAMVLEAE 436 Lambda K H 0.319 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 702 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 436 Length of database: 436 Length adjustment: 32 Effective length of query: 404 Effective length of database: 404 Effective search space: 163216 Effective search space used: 163216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate BWI76_RS20460 BWI76_RS20460 (acetyl-CoA C-acyltransferase FadI)
to HMM TIGR02446 (fadI: acetyl-CoA C-acyltransferase FadI (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02446.hmm # target sequence database: /tmp/gapView.30060.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02446 [M=430] Accession: TIGR02446 Description: FadI: acetyl-CoA C-acyltransferase FadI Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.7e-262 854.5 3.4 9.7e-262 854.3 3.4 1.0 1 lcl|FitnessBrowser__Koxy:BWI76_RS20460 BWI76_RS20460 acetyl-CoA C-acylt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Koxy:BWI76_RS20460 BWI76_RS20460 acetyl-CoA C-acyltransferase FadI # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 854.3 3.4 9.7e-262 9.7e-262 1 430 [] 7 436 .] 7 436 .] 1.00 Alignments for each domain: == domain 1 score: 854.3 bits; conditional E-value: 9.7e-262 TIGR02446 1 vktrqgeriaivaglrtpfarqatafhgipavdlgkmvvsellarseidaklieqlvfgqvvqmpeapnia 71 ++trqg+riaiv+glrtpfarqatafhg+pavdlgk+vv+e+lars i+a++ieqlvfgqvvqmpeapnia lcl|FitnessBrowser__Koxy:BWI76_RS20460 7 LVTRQGDRIAIVSGLRTPFARQATAFHGVPAVDLGKLVVGEMLARSTIPADIIEQLVFGQVVQMPEAPNIA 77 589******************************************************************** PP TIGR02446 72 reivlgtgmnvhtdaysvtracatsfqsavnvaesimagaieigiaggadsssvlpigvskklaralvdln 142 reivlgtgm vhtdaysv+racatsfq+++nvaes+mag+i++giaggadsssvlpigvskklar+lvd n lcl|FitnessBrowser__Koxy:BWI76_RS20460 78 REIVLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARTLVDAN 148 *********************************************************************** PP TIGR02446 143 kartlgqklkvlsrlglkdllpvppavaeystglsmgqtaeqmaktygitraeqdalahrshtlaaqawad 213 kartlgq+lk++srl+++dllpvppavaeystgl+mg+taeqmakt+gitr+ qdala rsh+laa+aw++ lcl|FitnessBrowser__Koxy:BWI76_RS20460 149 KARTLGQRLKLFSRLRFRDLLPVPPAVAEYSTGLRMGDTAEQMAKTWGITRERQDALALRSHQLAAKAWEE 219 *********************************************************************** PP TIGR02446 214 gklkeevmtafvepykkfiaednnirenssladyaklrpafdrkygsvtaanstpltdgaaavilmsegra 284 gkl evmta+++p+++++++dnnir+ns+lady klrpafdr++gsvtaanstpltdgaaavilm+e+ra lcl|FitnessBrowser__Koxy:BWI76_RS20460 220 GKLSAEVMTAYTPPFREPLEQDNNIRKNSTLADYQKLRPAFDRQHGSVTAANSTPLTDGAAAVILMTESRA 290 *********************************************************************** PP TIGR02446 285 kelgleilgyirsyaftaidveqdmllgpsyatplalkraglelsdltlidmheafaaqtlanvkllasek 355 kelgl++lgy+rsyaftaidv qdmllgp+++tplal+ragl+l dltlidmheafaaqtlan++ +as++ lcl|FitnessBrowser__Koxy:BWI76_RS20460 291 KELGLVPLGYLRSYAFTAIDVRQDMLLGPAWSTPLALERAGLTLADLTLIDMHEAFAAQTLANLHCMASDR 361 *********************************************************************** PP TIGR02446 356 faeeklgrakaigeidmskfnvlggsiayghpfaatgarlitqtlrelkrrggglglatacaagglgaami 426 fa+e lgr++aige+d+skfnvlggsiayghpfaatgar+itqtl+el+rrggg+gl+tacaagglg+am+ lcl|FitnessBrowser__Koxy:BWI76_RS20460 362 FAREVLGRSQAIGEVDESKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGSAMV 432 *********************************************************************** PP TIGR02446 427 leve 430 le+e lcl|FitnessBrowser__Koxy:BWI76_RS20460 433 LEAE 436 **97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (430 nodes) Target sequences: 1 (436 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 8.39 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory