GapMind for catabolism of small carbon sources

 

Aligments for a candidate for hpcD in Klebsiella michiganensis M5al

Align 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized)
to candidate BWI76_RS13115 BWI76_RS13115 2,3-dehydroadipyl-CoA hydratase

Query= BRENDA::A4YI89
         (259 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS13115 BWI76_RS13115
           2,3-dehydroadipyl-CoA hydratase
          Length = 255

 Score =  177 bits (449), Expect = 2e-49
 Identities = 101/252 (40%), Positives = 148/252 (58%), Gaps = 6/252 (2%)

Query: 10  KEGNLFWITLNRPDKLNALNAKLLEELDRAVSQAESDPEIRVIIITGKGKAFCAGADITQ 69
           +   +  +TLNRP   NALN  LL ++   +  A  DP + V +I+G  + F AGAD+ +
Sbjct: 8   RHARVLQLTLNRPQARNALNNALLMQIADVLDAAALDPTVGVCVISGNERFFAAGADLNE 67

Query: 70  F--NQLTPAEAWKFSKKGREIMDKIEALSKPTIAMINGYALGGGLELALACDIRIAAEEA 127
              N L PA       +   +  +I+A SKP IA +NGYALG G ELAL CD+ +A + A
Sbjct: 68  MAENDL-PATLDDIRPR---LWARIDAFSKPLIASVNGYALGAGCELALICDLIVAGDNA 123

Query: 128 QLGLPEINLGIYPGYGGTQRLTRVIGKGRALEMMMTGDRIPGKDAEKYGLVNRVVPLANL 187
           + GLPEI LG+ PG GGTQRL R +GK  A  M+++G+ I    A + GLV+ V P A  
Sbjct: 124 RFGLPEITLGMMPGAGGTQRLIRSVGKALASRMVLSGESIDAHQALRAGLVSEVYPPALT 183

Query: 188 EQETRKLAEKIAKKSPISLALIKEVVNRGLDSPLLSGLALESVGWGVVFSTEDKKEGVSA 247
           ++    LA  +A+ SP++L   K+ +    +  L +GL  E   + ++ +TED++EG+ A
Sbjct: 184 DEYALSLAATVARHSPLALRAAKQSLRLSQEVSLQAGLQQERQLFTLLSATEDRREGIDA 243

Query: 248 FLEKREPTFKGK 259
           FL KR   FKG+
Sbjct: 244 FLHKRTAEFKGR 255


Lambda     K      H
   0.315    0.135    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 114
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 259
Length of database: 255
Length adjustment: 24
Effective length of query: 235
Effective length of database: 231
Effective search space:    54285
Effective search space used:    54285
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory