GapMind for catabolism of small carbon sources

 

Alignments for a candidate for crr in Klebsiella michiganensis M5al

Align glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized)
to candidate BWI76_RS19555 BWI76_RS19555 PTS beta-glucoside transporter subunit EIIBCA

Query= CharProtDB::CH_088352
         (169 letters)



>FitnessBrowser__Koxy:BWI76_RS19555
          Length = 619

 Score =  126 bits (317), Expect = 6e-34
 Identities = 56/148 (37%), Positives = 92/148 (62%)

Query: 22  EIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIE 81
           +I+AP++G ++ ++ VPD  FA  ++G G+AI P+  K++AP  G +  IF+T HA  + 
Sbjct: 472 DILAPMTGSVLALDQVPDSTFASGLLGQGVAIIPSVGKVIAPFSGEVASIFQTKHAIGLL 531

Query: 82  SDSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVI 141
           SDSG+EL +H GIDTV+L G  F    +EG ++K GD ++EFD   + +      TP++I
Sbjct: 532 SDSGIELLIHVGIDTVKLDGAPFTAHVKEGDKIKAGDLLLEFDRQAILDAGYDLATPIII 591

Query: 142 SNMDEIKELIKLSGSVTVGETPVIRIKK 169
           SN D+ + L  +S S      P++ + +
Sbjct: 592 SNSDDFRTLDMVSASAVDAGQPLLSVSR 619


Lambda     K      H
   0.315    0.138    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 212
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 169
Length of database: 619
Length adjustment: 27
Effective length of query: 142
Effective length of database: 592
Effective search space:    84064
Effective search space used:    84064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory