Align Amino-acid ABC transporter, ATP-binding protein (characterized, see rationale)
to candidate BWI76_RS08095 BWI76_RS08095 arginine ABC transporter ATP-binding protein
Query= uniprot:Q88GX0 (260 letters) >FitnessBrowser__Koxy:BWI76_RS08095 Length = 241 Score = 276 bits (707), Expect = 2e-79 Identities = 141/243 (58%), Positives = 183/243 (75%), Gaps = 4/243 (1%) Query: 20 LIRIEGLNKHYGAFHVLRDIDLQVREGERIVLCGPSGSGKSTLIRCINRLEVAQQGSIQV 79 +I ++ ++K YG F VL D +V++GE +V+CGPSGSGKSTLI+ +N LE QQG I V Sbjct: 1 MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLIKTVNGLEPIQQGQIIV 60 Query: 80 DGIDLAATTREAAQVRSDIGMVFQHFNLFPHMSVLDNCLLAPTSVRGLSRKDAEERAR-- 137 DG + A++RS +GMVFQHF LFPH+S++DN LA V+ L+R A RA+ Sbjct: 61 DGTTVNDKKTNLAKLRSRVGMVFQHFELFPHLSIIDNLTLA--QVKVLNRDKAASRAKGL 118 Query: 138 MYLSKVGIESQAHKYPSQLSGGQQQRVAIARALCMKPRIMLFDEPTSALDPEMVAEVLDV 197 L +VG+ + A K+P+QLSGGQQQRVAIARALCM P MLFDEPTSALDPEM+ EVLDV Sbjct: 119 KLLERVGLSAHAEKFPAQLSGGQQQRVAIARALCMDPIAMLFDEPTSALDPEMINEVLDV 178 Query: 198 LVQLAGTGMTMLCVTHEMGFARQVAERVLFLEGGQIIEDSPPQVFFNQPRTERAKAFLAQ 257 +V+LA GMTM+ VTHEMGFAR+VA RV+F++ G+I+ED+ + FF P++ERAK FLA+ Sbjct: 179 MVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDADKEAFFANPQSERAKDFLAK 238 Query: 258 ILH 260 ILH Sbjct: 239 ILH 241 Lambda K H 0.322 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 186 Number of extensions: 4 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 260 Length of database: 241 Length adjustment: 24 Effective length of query: 236 Effective length of database: 217 Effective search space: 51212 Effective search space used: 51212 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory