Alignments for a candidate for bglF in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for bglF in Klebsiella michiganensis M5al

Align β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized)
to candidate BWI76_RS07290 BWI76_RS07290 PTS beta-glucoside transporter subunit EIIBCA

Query= TCDB::P08722
         (625 letters)



>FitnessBrowser__Koxy:BWI76_RS07290
          Length = 626

 Score =  375 bits (963), Expect = e-108
 Identities = 221/627 (35%), Positives = 338/627 (53%), Gaps = 22/627 (3%)

Query: 3   ELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVV 62
           E A KI+  VGGA+NI  L HCATRLR +  D +K     +   PG+I VVE GGQFQ+V
Sbjct: 5   ETADKILHAVGGAENIRMLTHCATRLRMEFNDRAKVNDAQIGTLPGVISVVEKGGQFQIV 64

Query: 63  IGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVYVISGIFTPLIGLMAATGILK 122
           IGN V  V+  +N      +      +N     ++ R + VIS  FTP+I  +   G++K
Sbjct: 65  IGNEVQQVYRRLNKALPEKKGPAGGDKNAKPAGIVARIISVISTTFTPVIPAITGAGMIK 124

Query: 123 GMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGYTAGKRFGGNPFTAMVIGGALV 182
            +LA+       +E S TY +L   +DA F+F P++L Y A  +F  NP  AM + G L+
Sbjct: 125 ALLAILKLTGVISETSSTYQLLNIIADAAFFFLPVLLAYGASLKFECNPILAMTLAGVLL 184

Query: 183 HPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPSAI 242
           HP I      G+      +DF+GI V L +Y+ SV+PII + WL S +ER      PS I
Sbjct: 185 HPGIGQMLAAGK-----AVDFVGINVLLSDYAGSVLPIILTVWLMSWVERFAEKVSPSII 239

Query: 243 KNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIFVM 302
           K F  P+L L++  P+  ++VGP    +++L+AAG   + +        +MG      ++
Sbjct: 240 KFFVKPMLILLITAPLALVVVGPAGILLNDLVAAGAGAIDRHASWLIPMLMGTLQPFLII 299

Query: 303 FGLHWGLVPLCINNFTVLGYDTMI-PLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAAL 361
            G  W + P+     +  GY+ +  P ++ + +A   A L V L  +++  + +A S+  
Sbjct: 300 TGTAWAMTPIATGQLSKSGYEMINGPGMLASNVAMGAATLCVALKTKNSNLRQLASSSGF 359

Query: 362 TSLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSF---GLPSIFTFMQT 418
           T+L GITEPA+YGV L  +   + A I G       G +    Y+F   GL ++  F+  
Sbjct: 360 TALLGITEPALYGVLLKFRRVLIAAMIGGGCAGVYAGVSGLVRYAFVSPGLAALPAFIGE 419

Query: 419 IPSTGIDFTVWASVIGGVIAIGCAFVGTVML-HFITAKRQPAQGAPQEKTPEVITPPEQG 477
            P          +++  ++    + V T  L  FI  K  P +     + P+    P+  
Sbjct: 420 NP---------MNIVHALVTCAISIVVTFTLTWFIAFKDVPEEQEEVGEAPQPAVSPKNE 470

Query: 478 G---ICSPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAI 534
           G   + SP+ G++V L  V D  F+ GLLG+G+AI P  G +R+P +G +     + HA+
Sbjct: 471 GETEVFSPLRGQVVALSEVNDDVFSGGLLGEGLAIRPEEGVLRAPFSGTVMMFLPSCHAV 530

Query: 535 GIESDDGVEILIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTP 594
           G++SD G+E+LIH+GIDTV L+G+ FS+ + VGD+V  G  LI FDIPAI +AG+DL TP
Sbjct: 531 GLQSDSGLELLIHIGIDTVNLNGQHFSSTLKVGDRVEAGQELIRFDIPAIEQAGYDLITP 590

Query: 595 VLISNSDDFTDVLPHGTAQISAGEPLL 621
           V++ N D+   +     AQ+  GE L+
Sbjct: 591 VIVVNGDEQHSLRLTAAAQVDYGEQLM 617


Lambda     K      H
   0.324    0.141    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1121
Number of extensions: 68
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 625
Length of database: 626
Length adjustment: 38
Effective length of query: 587
Effective length of database: 588
Effective search space:   345156
Effective search space used:   345156
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources