GapMind for catabolism of small carbon sources

 

Alignments for a candidate for malK1 in Klebsiella michiganensis M5al

Align MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized)
to candidate BWI76_RS17830 BWI76_RS17830 ABC transporter ATP-binding protein

Query= TCDB::Q9X103
         (369 letters)



>FitnessBrowser__Koxy:BWI76_RS17830
          Length = 364

 Score =  330 bits (845), Expect = 5e-95
 Identities = 182/375 (48%), Positives = 245/375 (65%), Gaps = 26/375 (6%)

Query: 3   MAQVVLENVTKVYENKVVAVKNANLVVEDKEFVVLLGPSGCGKTTTLRMIAGLEEITDGK 62
           M  VVL +V K Y +  V +K+ +L + D EF VL+GPSGCGK+T LRMIAGLEEI+ G+
Sbjct: 1   MGSVVLNSVRKSYGDAHV-IKDVSLTIPDGEFCVLVGPSGCGKSTLLRMIAGLEEISGGE 59

Query: 63  IYIDGKVVNDVEPKDRDIAMVFQNYALYPHMTVYENMAFGLKLRKYPKDEIDRRVREAAK 122
           ++I+ + V +VEPK RDIAMVFQ+YALYP MTV ENM F LK+ K PK EI+++V EAA 
Sbjct: 60  VHINERNVTEVEPKLRDIAMVFQSYALYPQMTVRENMGFALKMAKLPKAEINQKVNEAAA 119

Query: 123 ILGIENLLDRKPRQLSGGQRQRVAVGRAIVRNPKVFLFDEPLSNLDAKLRVQMRSELKKL 182
           +LG+E LL+R P+ LSGGQRQRVA+GRAIVR P+VFLFDEPLSNLDAKLR Q+R E+++L
Sbjct: 120 LLGLEPLLERLPKDLSGGQRQRVAMGRAIVRKPQVFLFDEPLSNLDAKLRTQVRGEIREL 179

Query: 183 HHRLQATIIYVTHDQVEAMTMADKIVVMKDGEIQQIGTPHEIYNSPANVFVAGFIGSPPM 242
           H RL+ T +YVTHDQ+EAMTM   IVV++DG I+Q GTP E+Y+ PAN+FVAGFIGSP +
Sbjct: 180 HRRLKTTSVYVTHDQIEAMTMGQMIVVLRDGRIEQAGTPLELYDRPANLFVAGFIGSPEI 239

Query: 243 NFVNARVVRG---------EGGLWIQASGFKVKVPKEFEDKLANYIDKEIIFGIRPEDIY 293
           N +   VV           +G L    +G +V               +++++ IRPE + 
Sbjct: 240 NQLPGEVVLNGNATSLRLKDGSLLALPAGLRVT------------DGQQVVYAIRPEQVN 287

Query: 294 DKLFALAPSPENTITGVVDVVEPLGSETILHVKVGDDLIVASVNPRTQAKEEQKIDLVLD 353
                +  + ++ +   V  VE  GS+  L    G     +    R   KE  KI L   
Sbjct: 288 ----VVHEARDDALAAKVTAVENTGSDMQLFCDTGGGAFTSVFKQRLAVKEGDKIWLQPK 343

Query: 354 MTRMHAFDKETEKAI 368
           ++ +H FD ++ + I
Sbjct: 344 LSGVHLFDAQSGQRI 358


Lambda     K      H
   0.319    0.138    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 362
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 369
Length of database: 364
Length adjustment: 30
Effective length of query: 339
Effective length of database: 334
Effective search space:   113226
Effective search space used:   113226
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory