Aligments for a candidate for malK_Ss in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for malK_Ss in Klebsiella michiganensis M5al

Align MalK, component of Maltose and maltooligosaccharide porter (characterized)
to candidate BWI76_RS26870 BWI76_RS26870 dipeptide ABC transporter ATP-binding protein

Query= TCDB::Q97UG5
         (617 letters)



>FitnessBrowser__Koxy:BWI76_RS26870
          Length = 327

 Score =  204 bits (519), Expect = 5e-57
 Identities = 109/317 (34%), Positives = 189/317 (59%), Gaps = 8/317 (2%)

Query: 7   SLLKVNELTAGYFNQDGFVIGVTNVNFEVYPNEIFAIAGESGCGKSTLAMAIYGLLKYPG 66
           +LL V++L+  + ++      V  V++ V   E+  I GESG GKS  ++AI GL+ YPG
Sbjct: 2   ALLNVDKLSVHFGDEGTPFRAVDRVSYSVNQGEVVGIVGESGSGKSVSSLAIMGLIDYPG 61

Query: 67  VVLRGHVYLKDKDILSITQEELRKLRMKEFVYVPQFAMDALDPVAKIGDQMMRAAVSHGV 126
            V+   +    +D+  I+++E R+L   E   + Q  M +L+P   +G Q+M A   H  
Sbjct: 62  RVMAEKLEFNGRDLKRISEKERRQLVGAEVAMIFQDPMTSLNPCYTVGYQIMEAIKVHQG 121

Query: 127 NVEEARKLIKEKLELVDL-----PYNVVNMYPHELSGGMRQRVVIATSILLNPSLIILDE 181
             ++ R  I+  ++L+ L     P + +++YPH+LSGGM QRV+IA +I   P L+I DE
Sbjct: 122 GNKKTR--IQRAIDLLTLVGIPDPASRLDVYPHQLSGGMSQRVMIAMAIACRPKLLIADE 179

Query: 182 PTTGLDVIVQYEILKDLKRIQRQLGVSLVIISHDISMLLMISDRVGIMYAGEIVEIGSKE 241
           PTT LDV +Q +I++ L  +Q++  ++L++I+HD++++   + ++ +MYAG++VE G  +
Sbjct: 180 PTTALDVTIQAQIIELLLELQQKENMALILITHDLALVAEAAHKIIVMYAGQVVETGDAK 239

Query: 242 EIIKRPSHPYTYLLISSLPSLVKRREKLLSIPGNPPLMLSKVPNSCRFYDRCPFKMEKCS 301
           +I + P HPYT  L+ +LP   + + +L S+PG  P    + P+ C    RCP+  +KC 
Sbjct: 240 DIFRAPRHPYTQALLRALPEFAQDKARLASLPGVVPGKYDR-PSGCLLNPRCPYATDKCR 298

Query: 302 TLNPALGDIMDGHKARC 318
           +  P L  +  G +++C
Sbjct: 299 SEEPELNLLNGGRQSKC 315



 Score =  156 bits (395), Expect = 1e-42
 Identities = 87/242 (35%), Positives = 142/242 (58%), Gaps = 7/242 (2%)

Query: 377 PINAVNDVSFELKKGTITALVGGSGHGKSTIAKILAGMIQQT----SGKIILLGKDVSEY 432
           P  AV+ VS+ + +G +  +VG SG GKS  +  + G+I       + K+   G+D+   
Sbjct: 19  PFRAVDRVSYSVNQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGRDLKRI 78

Query: 433 GVRNS-MWYKENVQMIFQDPYSSLDPRHTVRWHVERPLLIHKKVSNKDQLLPKIIEVLKN 491
             +         V MIFQDP +SL+P +TV + +   + +H+   NK   + + I++L  
Sbjct: 79  SEKERRQLVGAEVAMIFQDPMTSLNPCYTVGYQIMEAIKVHQG-GNKKTRIQRAIDLLTL 137

Query: 492 VGLKPPEKYLYKYPHELSGGERQRVAIARATAVEPKVLVADEPVSMLDASLRAGILNLIK 551
           VG+  P   L  YPH+LSGG  QRV IA A A  PK+L+ADEP + LD +++A I+ L+ 
Sbjct: 138 VGIPDPASRLDVYPHQLSGGMSQRVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLL 197

Query: 552 KF-KKNGISILYITHDIATVNYIADEIMVIYKGRIVEKGNTYEVISNPSHEYTKRLIEAV 610
           +  +K  ++++ ITHD+A V   A +I+V+Y G++VE G+  ++   P H YT+ L+ A+
Sbjct: 198 ELQQKENMALILITHDLALVAEAAHKIIVMYAGQVVETGDAKDIFRAPRHPYTQALLRAL 257

Query: 611 PD 612
           P+
Sbjct: 258 PE 259


Lambda     K      H
   0.319    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 502
Number of extensions: 27
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 617
Length of database: 327
Length adjustment: 33
Effective length of query: 584
Effective length of database: 294
Effective search space:   171696
Effective search space used:   171696
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources