Align alpha-glucosidase (EC 3.2.1.20) (characterized)
to candidate BWI76_RS06250 BWI76_RS06250 alpha-glycosidase
Query= BRENDA::P21517 (604 letters) >FitnessBrowser__Koxy:BWI76_RS06250 Length = 605 Score = 1040 bits (2690), Expect = 0.0 Identities = 476/604 (78%), Positives = 534/604 (88%) Query: 1 MLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDNEEMSVPMHKQRSQPQPG 60 ML AWHLPV PF+K+ +++L+ITLWL+G+D P R+ LR E DNEE+S+PMH+ R P PG Sbjct: 1 MLKAWHLPVAPFIKEQQERLMITLWLSGDDLPPRVTLRAEEDNEELSLPMHRLRQAPHPG 60 Query: 61 VTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMPPARLEQFAVDVPDIGPQWA 120 V AWR I+L +GQPRRRYSFKLLW DRQ WFTPQGF+R PPARLEQFAVD+PD GPQW Sbjct: 61 VVAWRGEINLVNGQPRRRYSFKLLWADRQLWFTPQGFNRFPPARLEQFAVDLPDSGPQWV 120 Query: 121 ADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILRDWDEPVTAQAGGSTFYGGDLD 180 ADQ+FYQIFPDRFARS REAEQD YYHHAAG +I+ + WDEP+TA+AGGSTFYGGDLD Sbjct: 121 ADQVFYQIFPDRFARSQSREAEQDATYYHHAAGHDIVRKAWDEPLTAEAGGSTFYGGDLD 180 Query: 181 GISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYRHVDPQFGGDGALLRLRHNTQQLG 240 GISEKLPYLK+LGVTALYLNPVF APSVHKYDTEDYR VDPQFGGD ALLRLRHNTQ+ G Sbjct: 181 GISEKLPYLKQLGVTALYLNPVFVAPSVHKYDTEDYRRVDPQFGGDAALLRLRHNTQKEG 240 Query: 241 MRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPESPWRDWYSFSDDGTALDWLGYASLPK 300 MRL+LDGVFNHSGDSHAWFDRH RG+GGACHN +SPWRDWY+FS +G A DWLGYASLPK Sbjct: 241 MRLILDGVFNHSGDSHAWFDRHQRGSGGACHNADSPWRDWYNFSPEGVAHDWLGYASLPK 300 Query: 301 LDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEA 360 LDY+S +L++EIY GEDS+VRHWLKAPW+MDGWRLDVVHMLGE GGARNN++H+AGIT+A Sbjct: 301 LDYRSSTLIDEIYGGEDSVVRHWLKAPWSMDGWRLDVVHMLGEGGGARNNLRHIAGITQA 360 Query: 361 AKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQT 420 AK +P+A++ GEHFGDARQWLQADVED+AMNYRGFTFPLWGFLANTDISYDPQ+IDAQT Sbjct: 361 AKLERPDAFVFGEHFGDARQWLQADVEDSAMNYRGFTFPLWGFLANTDISYDPQKIDAQT 420 Query: 421 CMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVPCIY 480 CMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFK+LLG+D+ARLPLAVVWLF+WPGVPCIY Sbjct: 421 CMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKSLLGKDVARLPLAVVWLFSWPGVPCIY 480 Query: 481 YGDEVGLDGKNDPFCRKPFPWQVEKQDTALFALYQRMIALRKKSQALRHGGCQVLYAEDN 540 YGDEVG+DG NDPFCRKPFPW QD L LY+RM LRK QALR+GGCQV+YAEDN Sbjct: 481 YGDEVGVDGNNDPFCRKPFPWDPALQDGDLLDLYKRMSKLRKAHQALRYGGCQVIYAEDN 540 Query: 541 VVVFVRVLNQQRVLVAINRGEACEVVLPASPFLNAVQWQCKEGHGQLTDGILALPAISAT 600 VVVFVRV QQRVLVAINRGEACEVV+ SP L+ WQ KEG G L DG+L LPAISA+ Sbjct: 541 VVVFVRVYKQQRVLVAINRGEACEVVIEDSPLLDVNGWQLKEGAGALHDGVLTLPAISAS 600 Query: 601 VWMN 604 VW + Sbjct: 601 VWFS 604 Lambda K H 0.322 0.138 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1571 Number of extensions: 62 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 604 Length of database: 605 Length adjustment: 37 Effective length of query: 567 Effective length of database: 568 Effective search space: 322056 Effective search space used: 322056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory