GapMind for catabolism of small carbon sources

 

Finding step cmtB for D-mannitol catabolism in Klebsiella michiganensis M5al

5 candidates for cmtB: mannitol phosphotransferase system, EII-A component CmtB/MtlF

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med BWI76_RS27160 PTS mannitol transporter subunit IICBA Mannitol-specific phosphotransferase enzyme IIA component, component of The mannitol porter (MtlA) (mannitol-1-P forming), MtlAF (characterized) 42% 96% 120.6 protein-Npi-phosphohistidine-D-mannitol phosphotransferase (EC 2.7.1.197) 94% 1165.2
med BWI76_RS19735 bifunctional PTS fructose transporter subunit IIA/HPr protein Mannitol-specific phosphotransferase enzyme IIA component, component of The mannitol porter (MtlA) (mannitol-1-P forming), MtlAF (characterized) 40% 97% 114 Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr 86% 636.3
med BWI76_RS20265 sugar phosphotransferase subunit IIA mannitol-specific PTS enzyme IIA component CmtB (EC 2.7.1.197) (characterized) 40% 98% 105.1 PTS system ascorbate-specific EIIA component; EC 2.7.1.- 40% 114.4
lo BWI76_RS24870 phosphocarrier, HPr family protein Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component (characterized) 37% 94% 110.9 PTS system tagatose-specific IIA-TPr component, component of The tagatose-specific PTS transporter/kinase, TagIIA-TPr/TagIIB'BC (tagatose-1-P forming) 85% 455.3
lo BWI76_RS17585 phosphoenolpyruvate-dependent PTS family enzyme IIA component Mannitol-specific phosphotransferase enzyme IIA component, component of The mannitol porter (MtlA) (mannitol-1-P forming), MtlAF (characterized) 31% 99% 92 Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr 34% 101.7

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.

Also see fitness data for the candidates

Definition of step cmtB

Or cluster all characterized cmtB proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory