GapMind for catabolism of small carbon sources

 

Aligments for a candidate for TM1747 in Klebsiella michiganensis M5al

Align TM1747, component of Probable mannose/mannoside porter. Induced by beta-mannan (Conners et al., 2005). Regulated by mannose-responsive regulator manR (characterized)
to candidate BWI76_RS09105 BWI76_RS09105 glutathione ABC transporter permease GsiC

Query= TCDB::Q9X269
         (341 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS09105 BWI76_RS09105 glutathione
           ABC transporter permease GsiC
          Length = 306

 Score =  212 bits (539), Expect = 1e-59
 Identities = 113/315 (35%), Positives = 190/315 (60%), Gaps = 12/315 (3%)

Query: 25  LKFLLKRLLTIAISMVVVIVITYVLMWLAPGNFFELQRVRDAIARVTTPDDPAYQATLKG 84
           L +++KRLL +  ++++V V+ ++ + + PG+   L    +A A+V              
Sbjct: 2   LNYVIKRLLGLIPTLLIVAVLVFLFVHMLPGDPARLIAGPEADAQVVGM----------- 50

Query: 85  FEERYGLNNPLWKQILMYLKGAVVFKFGPSFSDPARNIEDLIKEKFPITFTLALSSILFA 144
             ++ GL+ PL  Q   Y+   V   FG S +   R +   I  +F  TF L L+S+ +A
Sbjct: 51  VRQQLGLDRPLHVQFWHYISNIVQGDFGTSMAS-RRPVSSEIASRFMPTFWLTLASMSWA 109

Query: 145 LVVGVPLGILAALKKNTWIDYTAMTVSVIGVAIPSYVVAVFLILIFSIYLGWLPTSGWEG 204
           +V G+  GI+AA+ +N W D   M ++V G++ P++ + + L+ +FS+ LGWLPT G + 
Sbjct: 110 VVFGMAAGIVAAVWRNRWPDRLGMALAVTGISFPAFALGMLLMQVFSVELGWLPTVGADS 169

Query: 205 IRTKILPTIALALGPLASVARFTRVSLLDTLNQDFIRTAYAKGGDDRTVIMKHALRPSMI 264
            R  ILP++ L     A +ARFTR S +D L++D++RTA AKG  +  V++KH LR +MI
Sbjct: 170 WRHYILPSLTLGAAVAAVMARFTRASFVDVLHEDYMRTARAKGVSETLVVLKHGLRNAMI 229

Query: 265 PLVTIVGPQMAYLMVGTVWVENIFRIPGLGQLFANAAVTRDYPLLVTSTFILALTVMIMN 324
           P+VT++G Q  +L+ G++ VE +F  PGLG+L  ++   RDYP++     + +L  +++N
Sbjct: 230 PVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQAEVLLFSLEFILIN 289

Query: 325 LIVDVLYAILDPRIK 339
           L+VDVLYA ++P I+
Sbjct: 290 LVVDVLYAAINPAIR 304


Lambda     K      H
   0.328    0.143    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 261
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 341
Length of database: 306
Length adjustment: 28
Effective length of query: 313
Effective length of database: 278
Effective search space:    87014
Effective search space used:    87014
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory