Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate BWI76_RS20455 BWI76_RS20455 multifunctional fatty acid oxidation complex subunit alpha
Query= metacyc::MONOMER-20678 (699 letters) >FitnessBrowser__Koxy:BWI76_RS20455 Length = 714 Score = 339 bits (869), Expect = 3e-97 Identities = 234/708 (33%), Positives = 354/708 (50%), Gaps = 45/708 (6%) Query: 11 DQVAIVTVDSP--PVNALSAAVR---RGILENVNAAVADPAVQAIVLVCAGR-TFIAGAD 64 D +A++T+D+P +N L A RGI+ + + ++ V + A FIAGAD Sbjct: 14 DNIAVITIDAPGEKMNTLKAEFASEVRGIIRQIRD---NKELRGAVFISAKPDNFIAGAD 70 Query: 65 ITEFGK---PPQPPAL----NDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVK 117 I + + AL ++A + P IAAIHG LGGGLE+AL CH RV Sbjct: 71 INMIARCHSAQEAEALARQGQQIMAEIHGLSIPVIAAIHGACLGGGLELALACHGRVCSD 130 Query: 118 EAK--LGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVVE 175 + K LGLPEV+LGLLPG+GGTQRLPR +G A++MI+ G + +ALK GLV+EVV Sbjct: 131 DDKTRLGLPEVQLGLLPGSGGTQRLPRLIGVSGALEMILTGKQLRPRQALKAGLVDEVVA 190 Query: 176 NLVAGAVAFAKKVLAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMT--KKARGLEAPF 233 + + A ++ + RP R ++ A RS+ VA T K A Sbjct: 191 QTIL--LQTAVELALKGRPASRAVPARERILAGPLGRSLLFRLVAKKTHHKTQGNYPATE 248 Query: 234 ACADAIGAAIDLPFEEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPR 293 + + G +E F +L ++ QS+A R FFA + K G +P Sbjct: 249 RILQVMETGLAQGCSSGYAEEARAFGELAMTPQSQALRSIFFASTDLKKDRGAD--AEPV 306 Query: 294 PVSRVAIIGAGTMGGGIA-MSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLP 352 + V I+G G MGGGIA ++ G+PV + + + L + R L Sbjct: 307 ALRSVGILGGGLMGGGIAYVTACKGGLPVRIKDIQPRGINHALKYSWDLLDKQVRRRYLR 366 Query: 353 PDAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSY 412 +++ LI+G + + D++IEAVFE +A+K+++ + V+ H +P + ASNTS Sbjct: 367 ASERDRQIGLISGSLDYQGFAHRDVVIEAVFEDLALKQKMVSEVEQHCRPETIFASNTSS 426 Query: 413 LSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVV 472 L I EIAA RPQ V+G+HFFSP + M L E++ T + TAV +AK K P+V Sbjct: 427 LPIGEIAAQASRPQRVIGLHFFSPVDKMPLVEVIPHIGTDRQTIATAVKLAKLQGKTPIV 486 Query: 473 VGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIG-- 530 V GF NR+LA ++ +LL EG + +D + KFG P+GP + D G+D G Sbjct: 487 VADKAGFYVNRILAPYINEAMRLLMEGEPVEHIDNALVKFGFPVGPIQLLDEVGIDTGTK 546 Query: 531 --------WRSRKDRGIKSEIADALCEAGRFGQKTGKGYYKYEQGSR--APMPDPEVETL 580 W R + I ++ R G+K +G+Y Y R PDP + +L Sbjct: 547 IIPVLEAAWGERFSP--PANIISSILNDDRKGRKNNRGFYLYAAKGRKSKKRPDPAIYSL 604 Query: 581 INDTLAKLGLKRRDITDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIY 640 + + + ++++++ ER V M+NE AR +E++ D D+ ++G G+P + Sbjct: 605 LG-----ISSPQARLSEQQVAERCVMMMLNEAARCFDERVVRSARDGDIGAVFGIGFPPF 659 Query: 641 RGGPMHYADSVGLKHIAERLSAYAKATNDPSLEPAPLLARLAAEGKTF 688 GGP Y D++G +A L A A P P L +A +G TF Sbjct: 660 LGGPFRYMDTLGAGEVAAILQRLA-AQYGPRFTPCDTLLHMAEQGATF 706 Lambda K H 0.317 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1017 Number of extensions: 56 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 699 Length of database: 714 Length adjustment: 39 Effective length of query: 660 Effective length of database: 675 Effective search space: 445500 Effective search space used: 445500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory