Align pyruvate decarboxylase (EC 4.1.1.1) (characterized)
to candidate BWI76_RS20520 BWI76_RS20520 indolepyruvate decarboxylase
Query= BRENDA::P16467 (563 letters) >FitnessBrowser__Koxy:BWI76_RS20520 Length = 553 Score = 360 bits (925), Expect = e-104 Identities = 217/563 (38%), Positives = 308/563 (54%), Gaps = 21/563 (3%) Query: 5 TLGKYLFERLSQVNCNTVFGLPGDFNLSLLDKLYEVKGMRWAGNANELNAAYAADGYARI 64 T+G YL +RL + +FG+PGD+NL LD + + + W G ANELNAAYAADGYARI Sbjct: 6 TIGDYLLDRLVDCGIDRLFGVPGDYNLQFLDSVIAHRNLGWVGCANELNAAYAADGYARI 65 Query: 65 KGMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISSQAKQLLLHHTLGNGDFTV 124 KG ++TT+GVGELSALNGIAGSYAEHV VLH+VG P +Q + LLHHTLG+GDF+ Sbjct: 66 KGAGALLTTYGVGELSALNGIAGSYAEHVPVLHIVGAPCTGAQQRGELLHHTLGDGDFSH 125 Query: 125 FHRMSANISETTAMITDIANAPAEIDRCIRTTYTTQRPVYLGLPANLVDLNVPAKLLETP 184 F RMS I+ + A++ NA EIDR + T RP YL LPA++ AK TP Sbjct: 126 FSRMSEQITCSQAVLA-AGNACHEIDRVLSEMLTHHRPGYLMLPADV------AKAKTTP 178 Query: 185 ----IDLSLKPNDAEAEAEVVRTVVELIKDAKNPVILADACASRHDVKAETKKLMDLTQF 240 + + P D A +++ ++ +LAD A R+ ++ ++ + Sbjct: 179 PAHRLLIQGLPADENQLAGFREHAGRMLRSSRRVSLLADFLAQRYGLQNALREWVAKVPV 238 Query: 241 PVYVTPMGKGAIDEQHPRYGGVYVGTLSRPEVKKAVESADLILSIGALLSDFNTGSFSYS 300 MGKG DEQ + G Y G S E + A+E+AD I+ IG +D T F+ Sbjct: 239 AYATMLMGKGLFDEQQSGFVGTYSGIASAEETRDAIENADTIICIGTRFTDTITAGFTQH 298 Query: 301 YKTKNIVEFHSDHIKIRNATFPGVQMKFALQKLLDAIPEVVKDYKPVAVPARVPITKSTP 360 + +E +++ + F + M+ AL L++ + ++ V+ + P P Sbjct: 299 LPLEKTIEIQPFAVRVADRWFSRIPMEKALAILIELSASLAAEW--VSPNIQAPGVSGAP 356 Query: 361 ANTPMKQEWMWNHLGNFLREGDIVIAETGTSAFGINQTTFPTDVYAIVQVLWGSIGFTVG 420 + + Q+ WN + LR GDI++A+ GT+AFG P D IVQ LWGSIGFT+ Sbjct: 357 EGS-LTQKNFWNTVQKQLRPGDIILADQGTAAFGAAALKLPVDATLIVQPLWGSIGFTLP 415 Query: 421 ALLGATMAAEELDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYIFVLNNNGYTIEKLI 480 A GA +AA E +RV+L +GDG+ QLT+QE+ +M+R +P I +LNN GYT+E+ I Sbjct: 416 AAYGAQIAAAE----RRVVLIVGDGAAQLTIQELGSMLRDKQRPLILLLNNEGYTVERAI 471 Query: 481 HGPHAEYNEIQGWDHLALLPTFGAR-NYETHRVATTGEWEKLTQDKDFQDNSKIRMIEVM 539 HGP YN+I WD L F RV T E ++ D ++ ++EVM Sbjct: 472 HGPEQRYNDIALWDWNRLPDAFAPDVPSRCWRVTRTQELQEAMNSSVASD--RLTLVEVM 529 Query: 540 LPVFDAPQNLVKQAQLTAATNAK 562 LP D P L Q N++ Sbjct: 530 LPKMDIPDFLRTVTQALEERNSR 552 Lambda K H 0.318 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 763 Number of extensions: 29 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 563 Length of database: 553 Length adjustment: 36 Effective length of query: 527 Effective length of database: 517 Effective search space: 272459 Effective search space used: 272459 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory