GapMind for catabolism of small carbon sources

 

Aligments for a candidate for pad-dh in Klebsiella michiganensis M5al

Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate BWI76_RS03865 BWI76_RS03865 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase

Query= BRENDA::P05091
         (517 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS03865 BWI76_RS03865
           5-carboxymethyl-2-hydroxymuconate semialdehyde
           dehydrogenase
          Length = 488

 Score =  369 bits (946), Expect = e-106
 Identities = 199/469 (42%), Positives = 284/469 (60%), Gaps = 19/469 (4%)

Query: 54  FPTVNPSTGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIE 113
           F T NP+TGEV+ +VA G  ++V++AV AA+ AF     W  +    R RL+ RL DLI+
Sbjct: 19  FHTTNPATGEVLAEVASGGADEVNQAVAAAKEAFP---KWANLPMKERARLMRRLGDLID 75

Query: 114 RDRTYLAALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRH 173
           ++   +AA+ET D G P   +  V +        ++A    + +GKT P+D    +YT  
Sbjct: 76  QNVPEIAAMETADTGLPIHQTKNVLIPRASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLV 135

Query: 174 EPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPG 233
           +PVGVC  + PWN P +   WK+ P LA GN  V+K++E +PLTA  +  L  EAG P G
Sbjct: 136 QPVGVCALVSPWNVPFMTATWKVAPCLALGNTAVLKMSELSPLTADRLGELALEAGIPAG 195

Query: 234 VVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNI 293
           V+N+V G+G TAG A+  H DV  V+FTG T  GR I   AG   LK+ ++ELGGKSP +
Sbjct: 196 VLNVVQGYGATAGDALVRHHDVRAVSFTGGTATGRNIMKNAG---LKKYSMELGGKSPVL 252

Query: 294 IMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFD 353
           I  DAD++ A++ A F +F   G+ C AGSR F+Q+ IY EFV+R   RA    VG+P D
Sbjct: 253 IFEDADIERALDAALFTIFSINGERCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPTD 312

Query: 354 SKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADR----------GYFIQPTVF 403
             T+ G  + +  ++K+ GYI  G +EGA LL GG   AD+          G F++PTV 
Sbjct: 313 PNTQVGALISQQHWEKVSGYIRLGIEEGATLLAGG---ADKPTDLPAHLRNGNFLRPTVL 369

Query: 404 GDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQAL 463
            DV + M +A+EEIFGPV  +L FK   E +  AN+  YGLA+ ++T+D+ K   L++ +
Sbjct: 370 ADVDNRMRVAQEEIFGPVACLLPFKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGI 429

Query: 464 QAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKV 512
           +AG V+VN  +V   + PFGG K SG+GRE GEY  + + E+K V + +
Sbjct: 430 EAGMVFVNTQNVRDLRQPFGGVKASGTGREGGEYSFEVFAEMKNVCISM 478


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 606
Number of extensions: 23
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 488
Length adjustment: 34
Effective length of query: 483
Effective length of database: 454
Effective search space:   219282
Effective search space used:   219282
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory