GapMind for catabolism of small carbon sources

 

Aligments for a candidate for AZOBR_RS08235 in Klebsiella michiganensis M5al

Align L-proline and D-alanine ABC transporter, permease component 1 (characterized)
to candidate BWI76_RS05990 BWI76_RS05990 branched-chain amino acid ABC transporter permease

Query= reanno::azobra:AZOBR_RS08235
         (301 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS05990 BWI76_RS05990 branched-chain
           amino acid ABC transporter permease
          Length = 299

 Score =  223 bits (569), Expect = 3e-63
 Identities = 123/299 (41%), Positives = 182/299 (60%), Gaps = 10/299 (3%)

Query: 4   FLQQLINGLSLGAIYGLIAIGYTMVYGIIGMINFAHGEIYMIGAFVALITFLAIGSLGIT 63
           FLQQ++NG+SLG +Y LIAIGYTMVYG++ +INFAH ++ M+GAF  L  F +IG     
Sbjct: 6   FLQQVVNGMSLGGMYALIAIGYTMVYGVLRLINFAHADVMMVGAFTTLFLFSSIG----- 60

Query: 64  WVPLALLVMLVASMLFTAVYGWTVERIAYRPLRSSPRLAPLISAIGMSIFLQNYVQILQG 123
            +P  + V L  ++    ++G  ++R+AYRPLR + +++ LI+AIG+S FL+N   +L G
Sbjct: 61  -LPFGVAVFLTLALC--GLFGMLIDRVAYRPLRQASKISMLITAIGVSFFLENLFNVLFG 117

Query: 124 ARSKPLQPILPGNLTLMDGAVSVSYVRLATIVITIALMYGFTQLITRTSLGRAQRACEQD 183
             S+        N T   G V ++ V     +IT+ L+     L+ RT  G A RA   D
Sbjct: 118 GSSRFFSAPDFFNNTRAFGDVIITNVAWIVPLITVLLLLAILWLLYRTRYGMAIRAVAFD 177

Query: 184 KKMAGLLGVNVDRVISLTFVMGAALAAVAGMMVLLIYGVIDFYIGFLAGVKAFTAAVLGG 243
                L+G++ +R+ISL F +G++LAA+ G+   + Y  ID  +G L G+KAF AAVLGG
Sbjct: 178 VNTVRLMGIDANRIISLVFALGSSLAALGGVFYSISYPTIDPLMGVLIGLKAFAAAVLGG 237

Query: 244 IGSLPGAMLGGVVIGLIEAFWSGYMG--SEWKDVATFTILVLVLIFRPTGLLGRPEIEK 300
           IGS+ GA+LGG ++G  E            +KD   F  L+LVL+FRP G++G   +E+
Sbjct: 238 IGSVTGAVLGGFILGFTEVVAVALFPELGGYKDAFAFMFLILVLLFRPVGIMGDERLER 296


Lambda     K      H
   0.329    0.144    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 339
Number of extensions: 25
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 301
Length of database: 299
Length adjustment: 27
Effective length of query: 274
Effective length of database: 272
Effective search space:    74528
Effective search space used:    74528
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory