Align ABC-type branched-chain amino acid transport system, permease component protein (characterized, see rationale)
to candidate BWI76_RS07280 BWI76_RS07280 branched-chain amino acid ABC transporter permease
Query= uniprot:D8IUY4 (309 letters) >FitnessBrowser__Koxy:BWI76_RS07280 Length = 316 Score = 392 bits (1008), Expect = e-114 Identities = 199/316 (62%), Positives = 253/316 (80%), Gaps = 7/316 (2%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 MD IQQIING++LGS+YALIALGYTMVYG+L +INFAHGDILMVGA+ LS + + Sbjct: 1 MDTLIQQIINGVMLGSIYALIALGYTMVYGILRIINFAHGDILMVGALTTLSGMHALNAH 60 Query: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 P LP + QL +A++ A+ VC ++++ +ER AYR LRNAPRLAPLI+ IGVS+LLQT+AM Sbjct: 61 FPTLPPLAQLGLALLLAMTVCALLAMAVERFAYRRLRNAPRLAPLISGIGVSVLLQTVAM 120 Query: 121 MIWGRSPLPFPQVMPSDPVHIAG-------ALISPTQIMLLALAVLAMVGLVLIVEKTKM 173 ++W R+PL FPQ++P DP+ + ALI+ T I+ +ALA+ M+GL L+VE T++ Sbjct: 121 IVWSRNPLMFPQILPMDPIAVTHGSAAHPPALITVTGIVTVALALTVMLGLWLLVEYTRL 180 Query: 174 GRAMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGL 233 GR MRA AENPRIA LMGV+ N +I +TFAIG AA+AGVM A+NY +A F+MGF+PG+ Sbjct: 181 GRGMRAVAENPRIATLMGVNPNAIIALTFAIGGVFAALAGVMMASNYGSAGFSMGFLPGI 240 Query: 234 KAFSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVL 293 KAF+AAVLGGIGNI GAM+GG+LLGLIESLGAGY+GDLT GSNYQDIFAF+VLI+VL Sbjct: 241 KAFTAAVLGGIGNIRGAMIGGLLLGLIESLGAGYLGDLTHGVFGSNYQDIFAFMVLILVL 300 Query: 294 TLRPSGIMGERVADRA 309 RP+G++GERVA RA Sbjct: 301 VFRPAGLLGERVAHRA 316 Lambda K H 0.328 0.144 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 326 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 309 Length of database: 316 Length adjustment: 27 Effective length of query: 282 Effective length of database: 289 Effective search space: 81498 Effective search space used: 81498 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory