Aligments for a candidate for HSERO_RS00885 in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for HSERO_RS00885 in Klebsiella michiganensis M5al

Align ABC-type branched-chain amino acid transport system, permease component protein (characterized, see rationale)
to candidate BWI76_RS07280 BWI76_RS07280 branched-chain amino acid ABC transporter permease

Query= uniprot:D8IUY4
         (309 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS07280 BWI76_RS07280 branched-chain
           amino acid ABC transporter permease
          Length = 316

 Score =  392 bits (1008), Expect = e-114
 Identities = 199/316 (62%), Positives = 253/316 (80%), Gaps = 7/316 (2%)

Query: 1   MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60
           MD  IQQIING++LGS+YALIALGYTMVYG+L +INFAHGDILMVGA+  LS +  +   
Sbjct: 1   MDTLIQQIINGVMLGSIYALIALGYTMVYGILRIINFAHGDILMVGALTTLSGMHALNAH 60

Query: 61  APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120
            P LP + QL +A++ A+ VC ++++ +ER AYR LRNAPRLAPLI+ IGVS+LLQT+AM
Sbjct: 61  FPTLPPLAQLGLALLLAMTVCALLAMAVERFAYRRLRNAPRLAPLISGIGVSVLLQTVAM 120

Query: 121 MIWGRSPLPFPQVMPSDPVHIAG-------ALISPTQIMLLALAVLAMVGLVLIVEKTKM 173
           ++W R+PL FPQ++P DP+ +         ALI+ T I+ +ALA+  M+GL L+VE T++
Sbjct: 121 IVWSRNPLMFPQILPMDPIAVTHGSAAHPPALITVTGIVTVALALTVMLGLWLLVEYTRL 180

Query: 174 GRAMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGL 233
           GR MRA AENPRIA LMGV+ N +I +TFAIG   AA+AGVM A+NY +A F+MGF+PG+
Sbjct: 181 GRGMRAVAENPRIATLMGVNPNAIIALTFAIGGVFAALAGVMMASNYGSAGFSMGFLPGI 240

Query: 234 KAFSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVL 293
           KAF+AAVLGGIGNI GAM+GG+LLGLIESLGAGY+GDLT    GSNYQDIFAF+VLI+VL
Sbjct: 241 KAFTAAVLGGIGNIRGAMIGGLLLGLIESLGAGYLGDLTHGVFGSNYQDIFAFMVLILVL 300

Query: 294 TLRPSGIMGERVADRA 309
             RP+G++GERVA RA
Sbjct: 301 VFRPAGLLGERVAHRA 316


Lambda     K      H
   0.328    0.144    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 326
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 309
Length of database: 316
Length adjustment: 27
Effective length of query: 282
Effective length of database: 289
Effective search space:    81498
Effective search space used:    81498
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources