Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate BWI76_RS07630 BWI76_RS07630 high-affinity choline transporter BetT
Query= SwissProt::G3XCN6 (706 letters) >FitnessBrowser__Koxy:BWI76_RS07630 Length = 677 Score = 490 bits (1262), Expect = e-143 Identities = 257/656 (39%), Positives = 396/656 (60%), Gaps = 16/656 (2%) Query: 49 QRFKVNLPVFVGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFS 108 ++ K+N VF S +I LF + + + + + FGW YLL+ +++ Sbjct: 9 EKDKINPVVFYTSAGLILLFSLMTLFFSDFSAAWIGRTLNWVSRTFGWYYLLAATLYIVF 68 Query: 109 MLFLAFSRYGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASPPEAE 168 ++ +A SR+G +KLGP+ S+PEF LSW AMLFAAG+GI LM+F+V EP+T + PPE Sbjct: 69 VVCIACSRFGSVKLGPEHSKPEFSVLSWAAMLFAAGIGIDLMFFSVAEPVTQYMQPPEGA 128 Query: 169 PLTIAAQREAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKEGIHG 228 T+ A R+AM T FH+G+ W++Y+++G++L YF YRYNLPLT+RS LYP+ + I+G Sbjct: 129 GQTMEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYPIFGKKING 188 Query: 229 PIGHVVDIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAIATIS 288 PIGH VDI A+ GT+FG+AT+LG G++Q+N GL+ L IP S+ + L+ + IATIS Sbjct: 189 PIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFNIPDSLGAKAALIVLSVIIATIS 248 Query: 289 VVTGVEKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIYA 348 V +GV+KG+RILSE N+ LA+ L+LF+L +G T L+ V N+G Y++ + T N +A Sbjct: 249 VTSGVDKGIRILSELNVVLALGLILFILFMGDTEFLLNALVLNVGDYVNRFMGMTLNSFA 308 Query: 349 YE-PRPWIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFLWMTV 407 ++ P W+++WTLF+WAWW++WSPFVG+F+ARISRGRT+R+FV L +P FT LW++V Sbjct: 309 FDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVVGTLIIPFTFTLLWLSV 368 Query: 408 FGNTAIYVDTTIANGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVTSSDS 467 FGN+A+Y + N A + A + P ++++A + +F+VTS+DS Sbjct: 369 FGNSALY-EIIHGNAAFAAEAVAHPERGFYNLLAQYPAFTFSASVATITGLLFYVTSADS 427 Query: 468 GSLVIDTIAS---GGETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTALPFSL 524 G+LV+ S + P RIFW + G++ +L T G++ALQ+AT+ LPFS Sbjct: 428 GALVLGNFTSKLKDINSDAPNWLRIFWSVVIGLLTLGMLMTNGISALQNATVIMGLPFSF 487 Query: 525 VMLILVWSLFVGMRA-DLARTQSPGSLGPRAYPASG-VPWQRRLAMTLSTPDRRAVEKFL 582 V+ ++ L+ ++ D R + PR + W++RL+ ++ P R + + Sbjct: 488 VIFFVMAGLYKSLKVEDYRRESANRDTAPRPLGVQDRLSWKKRLSRLMNYPGTRYTRQMM 547 Query: 583 QASVLPALEAVARELTRR-------SRPASVGRDAETGALTLTVPAEGHRDFVYGVQMSE 635 + PA+E VA+EL R S P G G L L V ++FVY + + Sbjct: 548 ETVCFPAMEEVAQELKLRGAYVELKSLPPEEG--DSLGHLDLLVHMGDEQNFVYQIWPQQ 605 Query: 636 HKLPAFTAYDATVADVRYEARTFFSDGSRGYDIMGMADNQIINDVLFQFERYTGFV 691 + +P FT + Y TF +GS+G D+M + Q+I D+L Q+ER+ F+ Sbjct: 606 YSIPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFI 661 Lambda K H 0.326 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1285 Number of extensions: 73 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 677 Length adjustment: 39 Effective length of query: 667 Effective length of database: 638 Effective search space: 425546 Effective search space used: 425546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory