Alignments for a candidate for puuP in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuP in Klebsiella michiganensis M5al

Align Putrescine importer PuuP (characterized)
to candidate BWI76_RS10720 BWI76_RS10720 putrescine importer

Query= SwissProt::P76037
         (461 letters)



>FitnessBrowser__Koxy:BWI76_RS10720
          Length = 463

 Score =  838 bits (2164), Expect = 0.0
 Identities = 421/454 (92%), Positives = 439/454 (96%), Gaps = 1/454 (0%)

Query: 1   MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL 60
           MAIN+ L+ A++ GK RLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGIS+GHVPASYL
Sbjct: 1   MAINTSLDNASEAGKPRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISNGHVPASYL 60

Query: 61  LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL 120
           LALAGVLFTAISYGKLVRQFP+AGSAYTYAQKSI+PHVGFMVGWSSLLDYLFLPMINVLL
Sbjct: 61  LALAGVLFTAISYGKLVRQFPQAGSAYTYAQKSISPHVGFMVGWSSLLDYLFLPMINVLL 120

Query: 121 AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ 180
           AKIYLSALFP+VPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFI LVVQ
Sbjct: 121 AKIYLSALFPDVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIVLVVQ 180

Query: 181 GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK 240
           GLHKGEGVGTVWSLQPFIS+NAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
Sbjct: 181 GLHKGEGVGTVWSLQPFISQNAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK 240

Query: 241 AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN 300
           AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
Sbjct: 241 AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN 300

Query: 301 TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV 360
           TLASGLASHASVSRLLYVMGRDNVFPER+FGYVHPKWRTPALNVIMVGIVALSALFFDLV
Sbjct: 301 TLASGLASHASVSRLLYVMGRDNVFPERIFGYVHPKWRTPALNVIMVGIVALSALFFDLV 360

Query: 361 TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE 420
           TATALINFGALVAFTFVNLSVFNHFWRRKG NK+WKD FHYLL+P+VGALTVGVLW+NLE
Sbjct: 361 TATALINFGALVAFTFVNLSVFNHFWRRKGFNKTWKDRFHYLLLPMVGALTVGVLWINLE 420

Query: 421 STSLTLGLVWASLGGAYLWYLIRRYRK-VPLYDG 453
           +TSLTLGL+WA LG  YL YL RR+RK  P +DG
Sbjct: 421 ATSLTLGLIWAGLGLLYLTYLTRRFRKPPPQFDG 454


Lambda     K      H
   0.328    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 969
Number of extensions: 36
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 461
Length of database: 463
Length adjustment: 33
Effective length of query: 428
Effective length of database: 430
Effective search space:   184040
Effective search space used:   184040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources