Alignments for a candidate for puuP in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuP in Klebsiella michiganensis M5al

Align Low-affinity putrescine importer PlaP (characterized)
to candidate BWI76_RS18905 BWI76_RS18905 putrescine/spermidine ABC transporter

Query= SwissProt::P0AA47
         (452 letters)



>FitnessBrowser__Koxy:BWI76_RS18905
          Length = 452

 Score =  804 bits (2076), Expect = 0.0
 Identities = 398/452 (88%), Positives = 425/452 (94%)

Query: 1   MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVPTAYAFALI 60
           MSHN TP TSRVELRKTLTL+PVVMMGLAYMQPMTLFDTFGIVSGLTDGHV TAYAFALI
Sbjct: 1   MSHNATPKTSRVELRKTLTLIPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVATAYAFALI 60

Query: 61  AILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSSLLDYLFAPMINILLAKIY 120
           AILFTALSYGKLVRR+PSAGSAYTYAQKSISP VGFMVGWSSLLDYLF PMINILLAKIY
Sbjct: 61  AILFTALSYGKLVRRFPSAGSAYTYAQKSISPAVGFMVGWSSLLDYLFMPMINILLAKIY 120

Query: 121 FEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIVVLQVVLIAVILGMVVYGVFE 180
           FEALVPSIPSW+FVVALVAFMT  NLRS+K+VANFNT+IV+LQ+ ++AVI+G+++YGV  
Sbjct: 121 FEALVPSIPSWIFVVALVAFMTISNLRSIKTVANFNTLIVILQMGIVAVIVGLIIYGVMH 180

Query: 181 GEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFDGISNLSEETKDAERVIPRAIFL 240
           GEGAGTL STRPFWS  AHV+PMITGATILCFSF GFDGIS+LSEETKDAERVIP+AIFL
Sbjct: 181 GEGAGTLTSTRPFWSEGAHVVPMITGATILCFSFLGFDGISSLSEETKDAERVIPKAIFL 240

Query: 241 TALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEIMLYVAGKAFQVGALIFSTITVLAS 300
           TALIGG+IFI A+YFLQLYFPDISRFKDPDASQPEIMLYVAGK FQ G LIFS++TVLAS
Sbjct: 241 TALIGGLIFIGASYFLQLYFPDISRFKDPDASQPEIMLYVAGKTFQWGVLIFSSVTVLAS 300

Query: 301 GMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWRTPAMNIILVGAIALLAINFDLVMATA 360
           GMAAHAGV+RLMYVMGRDGVFP  FFGYVHPKWRTPA N++LVGAIALLAI FDLV ATA
Sbjct: 301 GMAAHAGVSRLMYVMGRDGVFPTRFFGYVHPKWRTPAWNVLLVGAIALLAIKFDLVTATA 360

Query: 361 LINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFQYLFLPMCGALTVGALWVNLEESSM 420
           LINFGALVAFTFVNLSVISQFWIREKRNKTLKDHF YL LP+CGALTVGALW+NLEESSM
Sbjct: 361 LINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFNYLILPVCGALTVGALWINLEESSM 420

Query: 421 VLGLIWAAIGLIYLACVTKSFRNPVPQYEDVA 452
           VLGLIW  IGL+YLACVTKSFRNPVPQYEDVA
Sbjct: 421 VLGLIWGGIGLVYLACVTKSFRNPVPQYEDVA 452


Lambda     K      H
   0.328    0.140    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 797
Number of extensions: 21
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 452
Length of database: 452
Length adjustment: 33
Effective length of query: 419
Effective length of database: 419
Effective search space:   175561
Effective search space used:   175561
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources