Alignments for a candidate for rhaT' in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Klebsiella michiganensis M5al

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate BWI76_RS00660 BWI76_RS00660 ABC transporter

Query= TCDB::Q7BSH4
         (512 letters)



>FitnessBrowser__Koxy:BWI76_RS00660
          Length = 503

 Score =  530 bits (1365), Expect = e-155
 Identities = 275/492 (55%), Positives = 360/492 (73%), Gaps = 2/492 (0%)

Query: 20  ILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEGEILV 79
           +L ++GI+++FPGV+AL+NV + L PG VTALIGENGAGKSTLVK++TGIY+P EGEIL 
Sbjct: 7   LLSLKGITKVFPGVRALENVQLDLWPGKVTALIGENGAGKSTLVKVMTGIYQPEEGEILY 66

Query: 80  DGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRF-RTIDWQTMNSRS 138
              P    + ++A   G+TAIHQETVLFDEL+V ENIF+G    T   + +DW  M+ ++
Sbjct: 67  KAIPIQLPNPESAHKVGITAIHQETVLFDELSVTENIFVGQYLHTGLLKKLDWPAMHRKA 126

Query: 139 KALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEIDDLFR 198
             +LT LE  IDP   LK LSIAQRH+VAIARALS EA++VI+DEPTAALS+ EI + ++
Sbjct: 127 SEILTRLEVQIDPRATLKTLSIAQRHMVAIARALSFEAQVVILDEPTAALSQHEILEFYQ 186

Query: 199 IVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEIVRMMVGRD 258
           IV  LK+ GKAILFISHKFDE++E+AD + +  R       G      ++ +V MMVGR 
Sbjct: 187 IVERLKQDGKAILFISHKFDEIFELADYYTIL-RDGVYVSSGAINDITEERMVAMMVGRA 245

Query: 259 VENVFPKIDVAIGGPVLEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSELSQSLFG 318
           +   +PK+D   G  VLE+++  H TEF  ISF+LRKGEILG YGL+GAGR+EL Q+L G
Sbjct: 246 ITQTYPKVDCIPGETVLEVKDLCHPTEFAHISFSLRKGEILGFYGLVGAGRTELMQALSG 305

Query: 319 ITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLARTS 378
           ++ P SG +VL+G+ +    P DAI AGIV VPEER + G  + +PI QN++LP L++ +
Sbjct: 306 VSHPSSGDIVLKGKPMRFRQPADAISAGIVCVPEERQKQGAIIALPIAQNISLPQLSKLN 365

Query: 379 RRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVIILDE 438
             G L  A E+ LA +YA RL ++A +    V TLSGGNQQKVVIGKWLAT P+VIILDE
Sbjct: 366 PGGVLNDAREWRLADEYASRLQVKAFSWRQAVETLSGGNQQKVVIGKWLATHPEVIILDE 425

Query: 439 PTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLSAGIFERAE 498
           PTKGIDIGSKAAVH F+SEL ++GL++IMVSSELPE++GM+DR++VM EGL    +   E
Sbjct: 426 PTKGIDIGSKAAVHQFMSELVSQGLAVIMVSSELPEVMGMADRIIVMHEGLMVAEYRAGE 485

Query: 499 LSPEALVRAATG 510
            + E +V AA+G
Sbjct: 486 ATAETIVSAASG 497



 Score = 69.7 bits (169), Expect = 2e-16
 Identities = 53/227 (23%), Positives = 107/227 (47%), Gaps = 8/227 (3%)

Query: 288 DISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGI 347
           ++   L  G++  + G  GAG+S L + + GI +P  G+++ +   I + +P+ A + GI
Sbjct: 25  NVQLDLWPGKVTALIGENGAGKSTLVKVMTGIYQPEEGEILYKAIPIQLPNPESAHKVGI 84

Query: 348 VYVPEERGRHGLALPMPIFQNMTLPSLARTSRRGFLRAANEFALARKYAERLDLRAAALS 407
             + +E     L   + + +N+ +     T   G L+  +  A+ RK +E L      + 
Sbjct: 85  TAIHQETV---LFDELSVTENIFVGQYLHT---GLLKKLDWPAMHRKASEILTRLEVQID 138

Query: 408 --VPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSI 465
               + TLS   +  V I + L+   +V+ILDEPT  +        +  +  L  +G +I
Sbjct: 139 PRATLKTLSIAQRHMVAIARALSFEAQVVILDEPTAALSQHEILEFYQIVERLKQDGKAI 198

Query: 466 IMVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEALVRAATGNA 512
           + +S +  EI  ++D   ++++G+        +++ E +V    G A
Sbjct: 199 LFISHKFDEIFELADYYTILRDGVYVSSGAINDITEERMVAMMVGRA 245


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 681
Number of extensions: 31
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 503
Length adjustment: 34
Effective length of query: 478
Effective length of database: 469
Effective search space:   224182
Effective search space used:   224182
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources