GapMind for catabolism of small carbon sources

 

D-ribose catabolism in Klebsiella michiganensis M5al

Best path

rbsA, rbsB, rbsC, rbsK

Also see fitness data for the top candidates

Rules

Overview: Ribose degradation in GapMind is based on the MetaCyc pathway ribose phosphorylation (link), which yields the central metabolic intermediate D-ribofuranose 5-phosphate, or on uptake by a phosphotransferase system.

14 steps (10 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
rbsA D-ribose ABC transporter, ATPase component RbsA BWI76_RS00275 BWI76_RS07240
rbsB D-ribose ABC transporter, substrate-binding component RbsB BWI76_RS00285 BWI76_RS07665
rbsC D-ribose ABC transporter, permease component RbsC BWI76_RS00280 BWI76_RS14865
rbsK ribokinase BWI76_RS00290 BWI76_RS00580
Alternative steps:
BT2809 D-ribose transporter
frcA D-ribose ABC transporter, ATPase component FrcA BWI76_RS14600 BWI76_RS14860
frcB D-ribose ABC transporter, substrate-binding component FrcB
frcC D-ribose ABC transporter, permease component FrcC BWI76_RS14865 BWI76_RS00280
fru2-IIA D-ribose PTS, IIA component BWI76_RS27580
fru2-IIB D-ribose PTS, IIB component BWI76_RS27575 BWI76_RS10665
fru2-IIC D-ribose PTS, IIC component BWI76_RS27570 BWI76_RS19725
LmGT2 D-ribose transporter LmGT2
PLT5 D-ribose transporter PLT5 BWI76_RS03110
rbsU probable D-ribose transporter RbsU

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory