GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Ac3H11_1695 in Klebsiella michiganensis M5al

Align ABC transporter permease (characterized, see rationale)
to candidate BWI76_RS05990 BWI76_RS05990 branched-chain amino acid ABC transporter permease

Query= uniprot:A0A165KC95
         (309 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS05990 BWI76_RS05990 branched-chain
           amino acid ABC transporter permease
          Length = 299

 Score =  211 bits (538), Expect = 1e-59
 Identities = 118/311 (37%), Positives = 184/311 (59%), Gaps = 22/311 (7%)

Query: 3   ILLQQIINGLVLGSMYALIALGYTMVYGIIQLINFAHGEVLMIGALTS---WSCIGMMQG 59
           I LQQ++NG+ LG MYALIA+GYTMVYG+++LINFAH +V+M+GA T+   +S IG+  G
Sbjct: 5   IFLQQVVNGMSLGGMYALIAIGYTMVYGVLRLINFAHADVMMVGAFTTLFLFSSIGLPFG 64

Query: 60  AMPGAPGWVILLLATIIACVVAATLNFVIEKVAYRPLRSSPRLAPLITAIGMSILLQTLA 119
                       +A  +   +      +I++VAYRPLR + +++ LITAIG+S  L+ L 
Sbjct: 65  ------------VAVFLTLALCGLFGMLIDRVAYRPLRQASKISMLITAIGVSFFLENLF 112

Query: 120 MIIWKPNYKPYPTM-LPSSPFEIGGAFITPTQILILGVTAVALASLVYLVNHTNLGRAMR 178
            +++  + + +      ++    G   IT    ++  +T + L ++++L+  T  G A+R
Sbjct: 113 NVLFGGSSRFFSAPDFFNNTRAFGDVIITNVAWIVPLITVLLLLAILWLLYRTRYGMAIR 172

Query: 179 ATAENPRVASLMGVKPDMVISATFIIGAVLAAIAGIMYASNYGTAQHTMGFLPGLKAFTA 238
           A A +     LMG+  + +IS  F +G+ LAA+ G+ Y+ +Y T    MG L GLKAF A
Sbjct: 173 AVAFDVNTVRLMGIDANRIISLVFALGSSLAALGGVFYSISYPTIDPLMGVLIGLKAFAA 232

Query: 239 AVFGGIGNLAGAVVGGILLGLIEAIGSGYIGTLTGGLLGSHYTDIFAFIVLIIILTLRPS 298
           AV GGIG++ GAV+GG +LG  E +       L G      Y D FAF+ LI++L  RP 
Sbjct: 233 AVLGGIGSVTGAVLGGFILGFTEVVAVALFPELGG------YKDAFAFMFLILVLLFRPV 286

Query: 299 GLLGERVADRA 309
           G++G+   +R+
Sbjct: 287 GIMGDERLERS 297


Lambda     K      H
   0.327    0.142    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 243
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 309
Length of database: 299
Length adjustment: 27
Effective length of query: 282
Effective length of database: 272
Effective search space:    76704
Effective search space used:    76704
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory