GapMind for catabolism of small carbon sources

 

Aligments for a candidate for braD in Klebsiella michiganensis M5al

Align High-affinity branched-chain amino acid transport system permease protein BraD, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate BWI76_RS07280 BWI76_RS07280 branched-chain amino acid ABC transporter permease

Query= TCDB::P21627
         (307 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS07280 BWI76_RS07280 branched-chain
           amino acid ABC transporter permease
          Length = 316

 Score =  243 bits (619), Expect = 6e-69
 Identities = 137/305 (44%), Positives = 187/305 (61%), Gaps = 12/305 (3%)

Query: 8   LQQLVNGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYIAFIAITLLAMMGLDS 67
           +QQ++NG+ +GS YALIA+GYTMVYGI+ +INFAHG++ M+G+      +  L       
Sbjct: 5   IQQIINGVMLGSIYALIALGYTMVYGILRIINFAHGDILMVGALTTLSGMHALNAHFPTL 64

Query: 68  VPLMMLA-AFAASIIVTSAFGYSIERVAYRPLRGGNRLIPLISAIGMSIFLQNAVMLSQD 126
            PL  L  A   ++ V +    ++ER AYR LR   RL PLIS IG+S+ LQ   M+   
Sbjct: 65  PPLAQLGLALLLAMTVCALLAMAVERFAYRRLRNAPRLAPLISGIGVSVLLQTVAMIVWS 124

Query: 127 SKEKAIPTLLPGNFV---FGESSMNGVVISYMQILIFVVTFLVMFGLTLFISRSRLGRAC 183
                 P +LP + +    G ++    +I+   I+   +   VM GL L +  +RLGR  
Sbjct: 125 RNPLMFPQILPMDPIAVTHGSAAHPPALITVTGIVTVALALTVMLGLWLLVEYTRLGRGM 184

Query: 184 RACAEDLKMTNLLGINSNNIIALTFVIGAALAAVAAVLLGMQYGVINPGIGFLAGIKAFT 243
           RA AE+ ++  L+G+N N IIALTF IG   AA+A V++   YG     +GFL GIKAFT
Sbjct: 185 RAVAENPRIATLMGVNPNAIIALTFAIGGVFAALAGVMMASNYGSAGFSMGFLPGIKAFT 244

Query: 244 AAVLGGIGSIPGAMLGGLLLGVAEAFGA--------DVFGDQYKDVVAFGLLILVLLFRP 295
           AAVLGGIG+I GAM+GGLLLG+ E+ GA         VFG  Y+D+ AF +LILVL+FRP
Sbjct: 245 AAVLGGIGNIRGAMIGGLLLGLIESLGAGYLGDLTHGVFGSNYQDIFAFMVLILVLVFRP 304

Query: 296 TGILG 300
            G+LG
Sbjct: 305 AGLLG 309


Lambda     K      H
   0.328    0.145    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 302
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 307
Length of database: 316
Length adjustment: 27
Effective length of query: 280
Effective length of database: 289
Effective search space:    80920
Effective search space used:    80920
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory