Align Dicarboxylate (succinate, fumarate, malate) transporter, vcINDY (characterized)
to candidate BWI76_RS24195 BWI76_RS24195 anion transporter
Query= TCDB::Q9KNE0 (462 letters) >FitnessBrowser__Koxy:BWI76_RS24195 Length = 462 Score = 739 bits (1907), Expect = 0.0 Identities = 364/461 (78%), Positives = 416/461 (90%) Query: 1 MNRNDSVPLPTNTREWFLHRNSLIVLADVALFLALYHFLPFEHNVVLGISMLAFIAVLWL 60 MN+ND PLPTNT EWF +RNS+I+L D+ LF+ LY+ LP+E VV+G+SMLAFIAVLWL Sbjct: 1 MNKNDMSPLPTNTHEWFFNRNSIIILLDIVLFIVLYNTLPYEPKVVMGLSMLAFIAVLWL 60 Query: 61 TEALHVTVTAILVPVMAVFFGIFETQAALNNFANSIIFLFLGGFALAAAMHHQGLDKVIA 120 TEALHVTVTAI+VPVMAV G+F TQAALN+FANS IFLFLGGFALAAAMH QGLDKVIA Sbjct: 61 TEALHVTVTAIMVPVMAVLLGVFNTQAALNSFANSTIFLFLGGFALAAAMHVQGLDKVIA 120 Query: 121 DKVLAMAQGKMSVAVFMLFGVTALLSMWISNTATAAMMLPLVLGVLSKVDADKQRSTYVF 180 DKVLAMA+GKMS+AVFMLFGVTALLSMWISNTATAAMM+PLVLG+LSKVD+ STYVF Sbjct: 121 DKVLAMARGKMSLAVFMLFGVTALLSMWISNTATAAMMMPLVLGILSKVDSKNSHSTYVF 180 Query: 181 VLLGVAYSASIGGIATLVGSPPNAIAAAEVGLSFTDWMKFGLPTAMMMLPMAIAILYFLL 240 VLLG+AYSASIGG+AT+VGSPPNAIAAAEVGLSF DWMKFG P +++LP+AIAILY + Sbjct: 181 VLLGIAYSASIGGMATIVGSPPNAIAAAEVGLSFFDWMKFGFPAMIVLLPVAIAILYLVF 240 Query: 241 KPTLNGMFELDRAPVNWDKGKVVTLGIFGLTVFLWIFSSPINAALGGFKSFDTLVALGAI 300 +P L G FE + V+WDKGK+VTL IF LTVF WIFS PIN LGGFKSFDT+VALGAI Sbjct: 241 RPELKGTFETNSERVDWDKGKIVTLAIFALTVFFWIFSGPINDLLGGFKSFDTIVALGAI 300 Query: 301 LMLSFARVVHWKEIQKTADWGVLLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFV 360 ++++FARVVHWK+I+KTADWGVLLLFGGG+CLSNVLK+TGTS+FLAN +S MV+HMGIF+ Sbjct: 301 ILVNFARVVHWKDIEKTADWGVLLLFGGGICLSNVLKETGTSLFLANQISGMVAHMGIFI 360 Query: 361 VILVVATFVVFLTEFASNTASAALLIPVFATVAEAFGMSPVLLSVLIAVAASCAFMLPVA 420 +ILV+ATFVVFLTEFASNTASAALLIPVFA+VAEAFGMSPV+LSVLIA+AASCAFMLPVA Sbjct: 361 IILVIATFVVFLTEFASNTASAALLIPVFASVAEAFGMSPVILSVLIAIAASCAFMLPVA 420 Query: 421 TPPNAIVFASGHIKQSEMMRVGLYLNIACIGLLTAIAMLFW 461 TPPNAIVFA+GHIKQ EMMR GL+LNIACI +LT +MLFW Sbjct: 421 TPPNAIVFATGHIKQQEMMRAGLFLNIACIIVLTGFSMLFW 461 Score = 39.7 bits (91), Expect = 2e-07 Identities = 37/147 (25%), Positives = 67/147 (45%), Gaps = 9/147 (6%) Query: 323 LLLFGGGLCLSNVLKQTGTSVFLANALSDMV-SHMGIFVVILVVATFVVFLTEFASNTAS 381 + LF GG L+ + G +A+ + M M + V +L T L+ + SNTA+ Sbjct: 97 IFLFLGGFALAAAMHVQGLDKVIADKVLAMARGKMSLAVFMLFGVT--ALLSMWISNTAT 154 Query: 382 AALLIPVFATV---AEAFGMSPVLLSVLIAVAASCAF---MLPVATPPNAIVFASGHIKQ 435 AA+++P+ + ++ + VL+ +A S + V +PPNAI A + Sbjct: 155 AAMMMPLVLGILSKVDSKNSHSTYVFVLLGIAYSASIGGMATIVGSPPNAIAAAEVGLSF 214 Query: 436 SEMMRVGLYLNIACIGLLTAIAMLFWQ 462 + M+ G I + + AI L ++ Sbjct: 215 FDWMKFGFPAMIVLLPVAIAILYLVFR 241 Lambda K H 0.329 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 753 Number of extensions: 38 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 462 Length of database: 462 Length adjustment: 33 Effective length of query: 429 Effective length of database: 429 Effective search space: 184041 Effective search space used: 184041 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory