Align β-fructohydrolase (ScrB) (intracellular) (EC 3.2.1.26) (characterized)
to candidate BWI76_RS07310 BWI76_RS07310 sucrose-6-phosphate hydrolase
Query= CAZy::AAS47898.1 (466 letters) >FitnessBrowser__Koxy:BWI76_RS07310 Length = 466 Score = 870 bits (2249), Expect = 0.0 Identities = 407/466 (87%), Positives = 422/466 (90%) Query: 1 MSLPSRLPAILQAVMQGQPRALADSHYPRWHHAPVTGLMNDPNGFIEFAGRYHLFYQWNP 60 MSLPSRLPAILQAVMQGQPRALADSHYPRWHHAPVTGLMNDPNGF+EFAGRYHLFYQWNP Sbjct: 1 MSLPSRLPAILQAVMQGQPRALADSHYPRWHHAPVTGLMNDPNGFVEFAGRYHLFYQWNP 60 Query: 61 LACDHTFKCWAHWSSIDLLHWQHEPIALMPDEEYDRNGCYSGSAVDNNGTLTLCYTGNVK 120 LACDH FKCWAHWSS DLL W+HEPIALMPDEEYDRNGCYSGSAVDNNGTLTLCYTGNVK Sbjct: 61 LACDHKFKCWAHWSSDDLLRWRHEPIALMPDEEYDRNGCYSGSAVDNNGTLTLCYTGNVK 120 Query: 121 FAEGGRTAWQCLATENADGTFRKIGPVLPLPEGYTGHVRDPKVWRHEDLWYMVLGAQDRQ 180 F +GGRTAWQCLATENADGTF K+GPVLPLPEGYTGHVRDPKVWRH+D WYMVLGAQDRQ Sbjct: 121 FDDGGRTAWQCLATENADGTFTKLGPVLPLPEGYTGHVRDPKVWRHQDRWYMVLGAQDRQ 180 Query: 181 KRGKVLLFSSADLHQWTSMGEIAGHGINGLDDVGYMWECPDLFPLGDQHILICCPQGIAR 240 KRGKVLLFSS DLHQW S GEIAG GINGL D GYMWECPDLF LGDQHILICCPQGIAR Sbjct: 181 KRGKVLLFSSPDLHQWRSEGEIAGDGINGLSDAGYMWECPDLFALGDQHILICCPQGIAR 240 Query: 241 EEECYLNTYPAVWMAGEFDYAAGAFRHGELHELDAGFEFYAPQTMLTSDGRRLLVGWMGV 300 EEE +LNTYPAVWMAG FDY AF HGEL ELDAGFEFYAPQT T DGRRLLVGWMGV Sbjct: 241 EEERFLNTYPAVWMAGGFDYDRAAFSHGELRELDAGFEFYAPQTTHTRDGRRLLVGWMGV 300 Query: 301 PEGEEMLQPTLNNGWIHQMTCLRELEFINGQLYQRPLRELSALRGEANGWSGNALPLAPM 360 P+GEEM QPTL +GWIHQMTCLRELEFI+GQLYQRPLREL+ALRGEA+GW GNALPLAPM Sbjct: 301 PDGEEMRQPTLAHGWIHQMTCLRELEFIDGQLYQRPLRELTALRGEAHGWQGNALPLAPM 360 Query: 361 EIDLQTRGGDMLSLDFGGVLTLECDASGLRLARRSLASDEMHYRYWRGNVRSLRVFIDQS 420 EI LQT D LSLDFGG LTLE DASG+RLARRSL SDEMHYRYWRG VR+LR+F D+S Sbjct: 361 EIALQTAADDALSLDFGGALTLERDASGIRLARRSLVSDEMHYRYWRGEVRTLRIFFDRS 420 Query: 421 SVEIFINGGEGVMSSRYFPACSGQLTFSGITPDAFCYWPLRTCMVE 466 SVEIFIN GEGVMSSR FPA QL FSG PDAFCYWPLRTCMVE Sbjct: 421 SVEIFINDGEGVMSSRCFPAYPAQLIFSGSAPDAFCYWPLRTCMVE 466 Lambda K H 0.322 0.139 0.468 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1060 Number of extensions: 46 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 466 Length of database: 466 Length adjustment: 33 Effective length of query: 433 Effective length of database: 433 Effective search space: 187489 Effective search space used: 187489 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory