Alignments for a candidate for ams in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for ams in Klebsiella michiganensis M5al

Align invertase (CscA) (EC 3.2.1.26) (characterized)
to candidate BWI76_RS23410 BWI76_RS23410 glycosyl hydrolase family 32

Query= CAZy::CAA57219.1
         (477 letters)



>FitnessBrowser__Koxy:BWI76_RS23410
          Length = 477

 Score =  562 bits (1448), Expect = e-164
 Identities = 267/478 (55%), Positives = 337/478 (70%), Gaps = 4/478 (0%)

Query: 1   MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLIWFNDRYHAFYQHHPMS 60
           MT S   A Q   AK HE     +YP +HLA  AGW+NDPNGL+WF+  YHAFYQHHP S
Sbjct: 1   MTYSIPRAEQELQAK-HEELNLRWYPRYHLAARAGWINDPNGLVWFDGWYHAFYQHHPYS 59

Query: 61  EHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSAVDDNGVLSLIYTGHVWLD 120
             WGPMHWGHA S D++HW+H P+ALAP    DKDGCFSGSAV D   L+LIYTGH +  
Sbjct: 60  TQWGPMHWGHARSKDLVHWEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKFHG 119

Query: 121 GAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWREADTWWMVVGAKDP 180
              ++  + +VQCLATSRDGIHFE+QG+++  P G+ HFRDPKVWRE D+W+M+VGA+D 
Sbjct: 120 DPSDEANLYQVQCLATSRDGIHFERQGIVVDTPPGMHHFRDPKVWREGDSWYMIVGARD- 178

Query: 181 GNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPDFFSLGDQHYLMFSPQGMNAEGY 240
           G+TGQ+ LYR + LR+W    VL  A+    YMWECPDFF+L  +H LMFSPQG+ A+GY
Sbjct: 179 GDTGQVRLYRSADLRQWQDAGVLDEAEKEMGYMWECPDFFALNGKHILMFSPQGLAAKGY 238

Query: 241 SYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFVAKDGRRIVIGWMDMWES 300
             RN FQSG + G W PG+ F + G F E+D+GHDFYAPQSF+  DGRRIVIGW+DMWES
Sbjct: 239 RNRNLFQSGYLLGEWQPGQAFVREGEFVEMDHGHDFYAPQSFLTPDGRRIVIGWLDMWES 298

Query: 301 PMPSKREGWAGCMTLARELS-ESNGKLLQRPVHEAESLRQQHQSISPRTISNKYVLQ-EN 358
           P+P +++GWAG ++L RELS  ++ +L  RP  E ES+R         T+ N+     EN
Sbjct: 299 PLPEQQDGWAGMLSLPRELSLSADNRLQMRPAKEVESMRGASFPWPVTTLKNQQTTTVEN 358

Query: 359 AQAVEIQLQWALKNSDAEHYGLQLGAGMRLYIDNQSERLVLWRYYPHENLDGYRSIPLPQ 418
            +A+E+ L W   +S AE YGL LG G+R+Y+D Q +RLVL R+YP   L G RS+PL +
Sbjct: 359 CEAMEVILHWDCASSSAEQYGLSLGDGLRVYVDAQMQRLVLERHYPQYGLCGTRSVPLTE 418

Query: 419 GDMLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELSLYASHGVAVLQHGALWQL 476
           G  L LRIF D+SSVEVF+NDGEA +SSRIYP    REL+L++  G A L+    WQL
Sbjct: 419 GADLKLRIFFDSSSVEVFVNDGEACLSSRIYPDAARRELALFSWSGSAYLKEAGAWQL 476


Lambda     K      H
   0.320    0.136    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 999
Number of extensions: 64
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 477
Length of database: 477
Length adjustment: 33
Effective length of query: 444
Effective length of database: 444
Effective search space:   197136
Effective search space used:   197136
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources