Align Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr (characterized)
to candidate BWI76_RS19735 BWI76_RS19735 bifunctional PTS fructose transporter subunit IIA/HPr protein
Query= SwissProt::P69811 (376 letters) >FitnessBrowser__Koxy:BWI76_RS19735 Length = 376 Score = 636 bits (1640), Expect = 0.0 Identities = 325/376 (86%), Positives = 355/376 (94%) Query: 1 MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI 60 MFQLSVQDIHPG++AG+KEEAIRQVA+ALV AGNVA+GYVNGMLAREQQTSTFLGNGIAI Sbjct: 1 MFQLSVQDIHPGQQAGNKEEAIRQVASALVSAGNVADGYVNGMLAREQQTSTFLGNGIAI 60 Query: 61 PHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDD 120 PHGTTDTRDQVLKTGVQV+QFP+GVTWG+GQ AYVAIGIAASSDEHLGLLRQLTHVLSDD Sbjct: 61 PHGTTDTRDQVLKTGVQVYQFPQGVTWGEGQTAYVAIGIAASSDEHLGLLRQLTHVLSDD 120 Query: 121 SVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIVASDLLTLQALNAARLKEAGA 180 SVAEQLKSATTAEELRALLMGEKQSE LKLDNE LTLD+ ASDLLTLQALNAARLKEAGA Sbjct: 121 SVAEQLKSATTAEELRALLMGEKQSEALKLDNETLTLDVAASDLLTLQALNAARLKEAGA 180 Query: 181 VDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN 240 VDA+FV++ IN++PLNLGQGIWL+DS EGNLRSA+AVSRAANAF D + ++L++VAM Sbjct: 181 VDASFVSRTINDKPLNLGQGIWLNDSTEGNLRSAVAVSRAANAFTSDEQPVSLLITVAMA 240 Query: 241 DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA 300 DDQP AVL RL++LLLD KADRLLKAD ATLLALLTSDDA +DVLSAE+V+RNEHGLHA Sbjct: 241 DDQPTAVLNRLSNLLLDKKADRLLKADGATLLALLTSDDAIAEDVLSAEYVIRNEHGLHA 300 Query: 301 RPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQA 360 RPGTMLVNTIKQF+SDITVTNLDG+GKPANGRSLMKVVALGVKKGHRLRFTAQG DA+QA Sbjct: 301 RPGTMLVNTIKQFSSDITVTNLDGSGKPANGRSLMKVVALGVKKGHRLRFTAQGEDAQQA 360 Query: 361 LKAIGDAIAAGLGEGA 376 L AIG+AIAAGLGEGA Sbjct: 361 LDAIGEAIAAGLGEGA 376 Lambda K H 0.314 0.130 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 561 Number of extensions: 10 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 376 Length adjustment: 30 Effective length of query: 346 Effective length of database: 346 Effective search space: 119716 Effective search space used: 119716 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory