Alignments for a candidate for ptsG in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for ptsG in Klebsiella michiganensis M5al

Align protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) (characterized)
to candidate BWI76_RS27535 BWI76_RS27535 PTS transporter subunit IIBC

Query= BRENDA::P69786
         (477 letters)



>FitnessBrowser__Koxy:BWI76_RS27535
          Length = 519

 Score =  226 bits (576), Expect = 1e-63
 Identities = 162/512 (31%), Positives = 256/512 (50%), Gaps = 51/512 (9%)

Query: 8   NLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVS-----HVMAEAGGS----VFAN 58
           N+QK  ++++  V  LP+ G++L + S   +  P ++S     H + +  G     +F N
Sbjct: 11  NMQKFSRAMIGAVLFLPVIGLILALSSVLTN--PTLISETSFLHQLGQLLGDTFWPLFGN 68

Query: 59  MPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIASKH------ 112
           + L+F +G++ G   +         V   IM            H  AE+I  ++      
Sbjct: 69  LGLLFCVGISYGLAKDKKTEVALVSVMCFIMFLGANHSWLEHTHGLAEKINGEYYGTGQT 128

Query: 113 ------LADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGV 166
                 + D GV  GII G   A++ N+   I+LP  L  + G +   +      IF  +
Sbjct: 129 QLLGFVVVDMGVFLGIILGCTIAWVHNKVSTIELPGALSMYGGAKLTLVAMTPVVIFYAI 188

Query: 167 VLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQM-QIGEY 225
             ++ WP +   I   + +         F +YGF E+ L+P GLHH    PFQ+ QIG  
Sbjct: 189 AFTWFWPFMTHGIAALTGFMKNAGVAGVF-VYGFFEKFLIPTGLHHFVWSPFQLTQIGGT 247

Query: 226 TNAAGQVFHGDIPRYMA----GDPT-----AGKLSGGFLFKMYGLPAAAIAIWHSAKPEN 276
            +  GQV  G    ++A     D T     A + S   +  ++GL  A++A +H+AKPE 
Sbjct: 248 LSVDGQVVSGTQAIFLAYMRHPDLTPVMNEALRFSQQGMTTIFGLAGASLAFYHTAKPEK 307

Query: 277 RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRD-GT 335
           +     I++ A +TS LTGITEPIEF+F+FV+P+L++IHA L   +  IC +  +R  G 
Sbjct: 308 KMMAKAILLPAIITSMLTGITEPIEFTFLFVSPLLWVIHATLTAASQAICDIFTVRPWGA 367

Query: 336 SFSHGLIDFIV----LSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGRED--- 388
           S   GLI+F++    L  + ++   + ++GIG   VYY IFR L+  L+LKTPGRED   
Sbjct: 368 S---GLIEFLIYNLPLPVSLTRWPGYVLIGIGQFAVYYVIFRTLVVKLNLKTPGREDDEN 424

Query: 389 ------ATEDAKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKK 442
                 A    K      +   ++   GGKENI ++D C TRLRV++ ++  VD   LK 
Sbjct: 425 VKLYSKADYRKKMGEPQSVTNEIINGLGGKENIISVDNCFTRLRVAIHNMDLVDDTILKS 484

Query: 443 LGAAGVVVAGSGVQAIFGTKSDNLKTEMDEYI 474
            GA GVV   + VQ I+G K   +++ +D ++
Sbjct: 485 TGANGVVRNRNEVQVIYGVKVGQVRSRVDSWL 516


Lambda     K      H
   0.325    0.140    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 667
Number of extensions: 45
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 477
Length of database: 519
Length adjustment: 34
Effective length of query: 443
Effective length of database: 485
Effective search space:   214855
Effective search space used:   214855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources