Alignments for a candidate for serP1 in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for serP1 in Klebsiella michiganensis M5al

Align Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized)
to candidate BWI76_RS02700 BWI76_RS02700 D-serine/D-alanine/glycine transporter

Query= TCDB::F2HQ25
         (459 letters)



>FitnessBrowser__Koxy:BWI76_RS02700
          Length = 467

 Score =  333 bits (855), Expect = 5e-96
 Identities = 182/454 (40%), Positives = 265/454 (58%), Gaps = 14/454 (3%)

Query: 2   ENLQEKHEAQRGLQNRHIQLIAIAGTIGTGLFLGAGKTIQMTGPSVIFAYILIGIAMFFF 61
           E    +   +R L NRHIQLIAI G IGTGLF+G+GKTI + GPS+IF Y++IG  +FF 
Sbjct: 11  EQAPTEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFV 70

Query: 62  LRTIGEMLYNDPSQHSFLNFVTKYSGVRTGYFTQWSYWLVIVFVCISELTAIGTYIQFWL 121
           +R +GE+L ++    SF +F     G   GYFT W+YW   V   ++++ AI  Y QFW 
Sbjct: 71  MRAMGELLLSNLEYKSFSDFAADLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWF 130

Query: 122 PQVPLWLIEIVMLALLFGLNTLNSRFFGETEFWFAMIKVAAIIGMIVTAIILVAGNFHYS 181
           P +  W+  + ++ LL GLN    + FGE EFWFAMIK+ AI+ +IV  +++V  +F   
Sbjct: 131 PGLSDWVASLAVILLLLGLNLATVKMFGEMEFWFAMIKIVAIVALIVVGLVMVMMHFQSP 190

Query: 182 TVLSGKTVHDSASLSNIFDGFQLFPHGAWNFVGALQMVMFAFTSMEFIGMTAAETVNPKK 241
           T +        AS +++++    FP G   F    Q+ +FAF  +E +G TAAET +P+K
Sbjct: 191 TGV-------EASFAHLWNDGGWFPKGISGFFAGFQIAVFAFVGIELVGTTAAETKDPEK 243

Query: 242 SLPKAINQIPVRILLFYVGALLAIMAIFNWHYIPADKSPFVMVFQLIGIKWAAALINFVV 301
           SLP+AIN IP+RI++FYV AL+ IM++  W  +   KSPFV +F L+G+  AA+LINFVV
Sbjct: 244 SLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPSKSPFVELFVLVGLPAAASLINFVV 303

Query: 302 LTSAASALNSSLFSATRNMYSLAQQHDKGRLTPFTKLSKAGIPINALYMATALSLLAPVL 361
           LTSAAS+ NS +FS +R ++ LAQ     ++  F KLSK  +P   L   + + LL  V+
Sbjct: 304 LTSAASSANSGVFSTSRMLFGLAQDGQAPKM--FAKLSKRAVPAKGLTF-SCMCLLGGVV 360

Query: 362 TLI--PQIKNAFDFAASCTTNLFLVVYFITLYTYWQYRKSED--YNPKGFLTPKPQITVP 417
            L+  P +  AF    + +  LF+ V+ I L +Y  YRK     +    +  P  ++   
Sbjct: 361 MLMVNPSVIAAFTMITTVSAILFMFVWTIILCSYLVYRKKRPQLHEQSKYKMPLGKLMCW 420

Query: 418 FIVAIFAIVFASLFFNADTFYPALGAIVWTIFFG 451
             +A FA V   L   ADT    +   +W +  G
Sbjct: 421 VCMAFFAFVLVLLTLEADTREALMVTPLWFVLLG 454


Lambda     K      H
   0.329    0.141    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 633
Number of extensions: 28
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 459
Length of database: 467
Length adjustment: 33
Effective length of query: 426
Effective length of database: 434
Effective search space:   184884
Effective search space used:   184884
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources