Aligments for a candidate for malK in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for malK in Klebsiella michiganensis M5al

Align MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized)
to candidate BWI76_RS26290 BWI76_RS26290 glycerol-3-phosphate ABC transporter ATP-binding protein

Query= TCDB::Q8DT25
         (377 letters)



>FitnessBrowser__Koxy:BWI76_RS26290
          Length = 356

 Score =  321 bits (822), Expect = 2e-92
 Identities = 186/377 (49%), Positives = 238/377 (63%), Gaps = 24/377 (6%)

Query: 1   MTTLKLDNIYKRYPNAKHYSVENFNLDIHDKEFIVFVGPSGCGKSTTLRMIAGLEDITEG 60
           M  LKL  + K + + K   ++   LD+ D EFIV VGPSGCGKST LRM+AGLE +T G
Sbjct: 1   MAGLKLQAVTKSW-DGKTQVIQPLTLDVADGEFIVMVGPSGCGKSTLLRMVAGLERVTSG 59

Query: 61  NLYIDDKLMNDASPKDRDIAMVFQNYALYPHMSVYENMAFGLKLRKYKKDDINKRVHEAA 120
           +++ID K + +  PKDR IAMVFQNYALYPHMSV ENMA+GLK+R   K  I +RV EAA
Sbjct: 60  DIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGLGKGLIAERVQEAA 119

Query: 121 EILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMDEPLSNLDAKLRVAMRAEIAK 180
            IL L   L+R+P +LSGGQRQRVAMGRAIVRD  VFL DEPLSNLDAKLRV MR E+  
Sbjct: 120 RILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQH 179

Query: 181 IHRRIGATTIYVTHDQTEAMTLADRIVIMSATPNPDKTGSIGRIEQIGTPQELYNEPANK 240
           +HRR+  T++YVTHDQ EAMTLA R+++M+           G  EQIGTP E+Y +PA++
Sbjct: 180 LHRRLKTTSLYVTHDQVEAMTLAQRVMVMNK----------GVAEQIGTPVEVYEKPASR 229

Query: 241 FVAGFIGSPAMNFFEVTVEKERLVNQDGLSLALPQGQEKIL--EEKGYLGKKVTLGIRPE 298
           FVA FIGSPAMN        E  V+ DG    L  G    +  E + Y G+K+TLGIRPE
Sbjct: 230 FVASFIGSPAMNLL------EGRVSDDGSRFELAGGMLLPINSEHRRYAGRKMTLGIRPE 283

Query: 299 DISSDQIVHETFPNASVTADILVSELLGSESMLYVKFGSTEFTARVNARDSHSPGEKVQL 358
             +         P   +  D L  E+LG++++ + ++G  +   R+  +     G  + L
Sbjct: 284 HFALSSQAEGGVP---LVMDTL--EILGADNLAHGRWGEQKLVVRLPHQQRPQAGSTLWL 338

Query: 359 TFNIAKGHFFDLETEKR 375
                  H FD ET +R
Sbjct: 339 HLPQESLHLFDSETGQR 355


Lambda     K      H
   0.318    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 336
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 377
Length of database: 356
Length adjustment: 30
Effective length of query: 347
Effective length of database: 326
Effective search space:   113122
Effective search space used:   113122
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources