Align TreT, component of Trehalose porter (characterized)
to candidate BWI76_RS26300 BWI76_RS26300 glycerol-3-phosphate transporter permease
Query= TCDB::Q97ZC2 (275 letters) >FitnessBrowser__Koxy:BWI76_RS26300 Length = 295 Score = 104 bits (259), Expect = 3e-27 Identities = 87/274 (31%), Positives = 135/274 (49%), Gaps = 19/274 (6%) Query: 9 FLLVLPALAYVISFAFFPTIEAVYLSFQ--DPHGGFSLY----NYKEL---SYF--NLSS 57 +LLV P L + F +P EA++ S Q DP G S + N+ L Y+ + + Sbjct: 15 YLLVAPQLVITVIFFIWPAGEALWYSLQSVDPFGLSSQFVGLDNFLALFQDPYYLDSFWT 74 Query: 58 AIINTIVVTIGALAIQLALGFLVASVLSREFFGKRALSTITIIPMGIATVVAAVTFSFVF 117 I + +VT+ L I L LV V+ G R T+ ++P +A VAAV + F+F Sbjct: 75 TIKFSTMVTVSGLLISLFFAALVDYVVR----GSRFYQTLMLLPYAVAPAVAAVLWIFLF 130 Query: 118 QTSGGYANTILHSLFGLNVNWYQSSISSLLVVMIADSWKNTPIVALILLAGMSSIPKELY 177 G +L + G + N Q+S ++ +V+ A WK L A + SIP+ L Sbjct: 131 NPGRGLITHLLGEM-GYDWNHAQNSGQAMFLVVFASVWKQISYNFLFFFAALQSIPRSLV 189 Query: 178 YASAIDGAGPIRRFFYITLPNLRSFIGISLILRGVQE-FNIFALPLILIGEHP-PLLTTL 235 A+AIDGAGP+RRFF + LP + L++ V F+ F + G P TTL Sbjct: 190 EAAAIDGAGPVRRFFRLALPLIAPVSFFLLVVNLVYAFFDTFPVIDAATGGGPVQATTTL 249 Query: 236 IYDLYTTTFPEVGL-ALASATILLGFILVFSGIV 268 IY +Y F + L A A+ +++L F+++ +V Sbjct: 250 IYKIYREGFAGLDLSASAAQSVVLMFLVIILTVV 283 Lambda K H 0.328 0.143 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 204 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 275 Length of database: 295 Length adjustment: 26 Effective length of query: 249 Effective length of database: 269 Effective search space: 66981 Effective search space used: 66981 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory