Align Muconolactone isomerase (EC 5.3.3.4) (characterized)
to candidate BWI76_RS13695 BWI76_RS13695 muconolactone delta-isomerase
Query= reanno::WCS417:GFF4625 (96 letters) >FitnessBrowser__Koxy:BWI76_RS13695 Length = 96 Score = 129 bits (324), Expect = 8e-36 Identities = 59/96 (61%), Positives = 71/96 (73%) Query: 1 MLFHVKMTVNLPLDMNPERAAGLKAEEKALAQRLQQEGKWRHLWRIAGHYANYSVFDVDS 60 MLF V MTVN+PL + +K EK +Q+LQ+EGKWRH+WR+AG YAN S+FDV Sbjct: 1 MLFKVDMTVNIPLGFPADEIEEIKLREKNYSQQLQREGKWRHIWRVAGLYANVSIFDVKD 60 Query: 61 VQELHDLLMQLPLYPYMAIEVNALCRHPSSIHEDDR 96 +ELH +LM LPLYP+M I V ALCRHPSSI EDDR Sbjct: 61 AEELHQILMALPLYPFMQIRVEALCRHPSSIREDDR 96 Lambda K H 0.322 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 58 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 96 Length of database: 96 Length adjustment: 10 Effective length of query: 86 Effective length of database: 86 Effective search space: 7396 Effective search space used: 7396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.0 bits) S2: 39 (19.6 bits)
Align candidate BWI76_RS13695 BWI76_RS13695 (muconolactone delta-isomerase)
to HMM TIGR03221 (catC: muconolactone delta-isomerase (EC 5.3.3.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03221.hmm # target sequence database: /tmp/gapView.22696.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03221 [M=90] Accession: TIGR03221 Description: muco_delta: muconolactone delta-isomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-46 142.4 0.0 2e-46 142.3 0.0 1.0 1 lcl|FitnessBrowser__Koxy:BWI76_RS13695 BWI76_RS13695 muconolactone delt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Koxy:BWI76_RS13695 BWI76_RS13695 muconolactone delta-isomerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 142.3 0.0 2e-46 2e-46 1 90 [] 2 91 .. 2 91 .. 0.99 Alignments for each domain: == domain 1 score: 142.3 bits; conditional E-value: 2e-46 TIGR03221 1 lflvrmdvnlPedlpaekaaelkaeekalaqelqreGkwrhlwrvvGeyanvsifdvesndelhellsglPlf 73 lf+v m+vn+P +pa++++e+k +ek+++q+lqreGkwrh+wrv+G yanvsifdv++ +elh++l++lPl+ lcl|FitnessBrowser__Koxy:BWI76_RS13695 2 LFKVDMTVNIPLGFPADEIEEIKLREKNYSQQLQREGKWRHIWRVAGLYANVSIFDVKDAEELHQILMALPLY 74 8************************************************************************ PP TIGR03221 74 pymdievtalarhPsai 90 p+m+i+v+al+rhPs+i lcl|FitnessBrowser__Koxy:BWI76_RS13695 75 PFMQIRVEALCRHPSSI 91 ***************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (90 nodes) Target sequences: 1 (96 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 4.23 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory