GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mhpD in Klebsiella michiganensis M5al

Align 2-oxopent-4-enoate hydratase (EC 4.2.1.80) (characterized)
to candidate BWI76_RS16910 BWI76_RS16910 2-keto-4-pentenoate hydratase

Query= BRENDA::P77608
         (269 letters)



>FitnessBrowser__Koxy:BWI76_RS16910
          Length = 268

 Score =  442 bits (1138), Expect = e-129
 Identities = 217/259 (83%), Positives = 235/259 (90%)

Query: 1   MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHINVQHDVAQGRRVVGRK 60
           MT  +LEQLA  LR A  +G+AI PLRD +G+DNAEAAYAIQ +NVQH VA GRRVVGRK
Sbjct: 1   MTTLSLEQLAQRLRDAETRGQAIEPLRDTLGVDNAEAAYAIQRMNVQHHVANGRRVVGRK 60

Query: 61  VGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQPRIEAEIALVLNRDLPATD 120
           VGLTHPKVQQQLGVDQPDFGTLFADMCYGDN  +PFSRVLQP++EAEIAL+L RDLP  D
Sbjct: 61  VGLTHPKVQQQLGVDQPDFGTLFADMCYGDNAEVPFSRVLQPKVEAEIALLLKRDLPHAD 120

Query: 121 ITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNASCGVYVIGGPAQRPAGLDLKN 180
            TFDELY AI+WVLPALEVVGSRIRDWSI FVDTVADNASCGVYV+G PA+RP GLDLKN
Sbjct: 121 TTFDELYAAIDWVLPALEVVGSRIRDWSIGFVDTVADNASCGVYVLGSPARRPDGLDLKN 180

Query: 181 CAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASLGEPLRTGDIILTGALGPMVAVN 240
           CAM+MTRN EEVSSGRGSECLGHPLNAAVWLARKMASLGEPLR GDI+LTGALGPMVA+N
Sbjct: 181 CAMRMTRNEEEVSSGRGSECLGHPLNAAVWLARKMASLGEPLRAGDIVLTGALGPMVAIN 240

Query: 241 AGDRFEAHIEGIGSVAATF 259
           AGDRFEAHI+G+GSVAATF
Sbjct: 241 AGDRFEAHIDGLGSVAATF 259


Lambda     K      H
   0.319    0.135    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 346
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 269
Length of database: 268
Length adjustment: 25
Effective length of query: 244
Effective length of database: 243
Effective search space:    59292
Effective search space used:    59292
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory