GapMind for catabolism of small carbon sources

 

Finding step praB for L-tryptophan catabolism in Klebsiella michiganensis M5al

5 candidates for praB: 2-hydroxymuconate 6-semialdehyde dehydrogenase

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med BWI76_RS03865 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 46% 98% 433 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 97% 959.1
med BWI76_RS07615 betaine-aldehyde dehydrogenase 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 42% 97% 382.5 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 87% 860.1
med BWI76_RS21985 aldehyde dehydrogenase 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 42% 97% 368.2 acetaldehyde dehydrogenase (EC 1.2.1.3) 100% 1020.8
med BWI76_RS10695 aldehyde dehydrogenase PuuC 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 41% 100% 364.8 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) 82% 824.7
lo BWI76_RS05620 NAD-dependent succinate-semialdehyde dehydrogenase aminomuconate-semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 37% 95% 293.5 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 89% 860.5

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step praB

Or cluster all characterized praB proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory