Align Leu/Ile/Val-binding protein LivJ aka B3460 aka LIV-BP, component of Leucine; leucine/isoleucine/valine porter (characterized)
to candidate BWI76_RS26350 BWI76_RS26350 branched chain amino acid ABC transporter substrate-binding protein
Query= TCDB::P0AD96 (367 letters) >FitnessBrowser__Koxy:BWI76_RS26350 Length = 369 Score = 563 bits (1451), Expect = e-165 Identities = 276/369 (74%), Positives = 317/369 (85%), Gaps = 2/369 (0%) Query: 1 MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQEFTGAEQAVADINAKG 60 M K ++AG +ALA S A A+DIKVAVVGAMSGPVAQ+GD EF GA QA+ DINA+G Sbjct: 1 MKRNAKTVIAGLVALAMSQAAFAKDIKVAVVGAMSGPVAQWGDMEFNGARQAIKDINAQG 60 Query: 61 GIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCSSSTQPASDIYEDEGILMI 120 GIKG+KL V+YDDACDPKQAVAVANK+VNDGI+YVIGHLCSSSTQPASDIYEDEGILMI Sbjct: 61 GIKGDKLVAVEYDDACDPKQAVAVANKIVNDGIQYVIGHLCSSSTQPASDIYEDEGILMI 120 Query: 121 TPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVKPQRIAIVHDKQQYGEGLARA 180 +P AT PELT RGYQ I+RT GLDS QGPTAAKYI+E VKPQRIAI+HDKQQYGEGLAR+ Sbjct: 121 SPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYIMETVKPQRIAIIHDKQQYGEGLARS 180 Query: 181 VQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAG 240 VQ+ LKKG AN+VFFDGITAGEKDFS L+ARLKKENIDFVYYGGY+PEMGQ+LRQAR+ G Sbjct: 181 VQESLKKGGANIVFFDGITAGEKDFSALLARLKKENIDFVYYGGYYPEMGQMLRQARSVG 240 Query: 241 LKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFV 300 LKT FMGPEGV N SLSNIAG++AEG+LVT PK YDQ PAN IVDA+KA+K+DPSG +V Sbjct: 241 LKTVFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYDQDPANSAIVDALKAEKKDPSGPYV 300 Query: 301 WTTYAALQSLQAGLNQ--SDDPAEIAKYLKANSVDTVMGPLTWDEKGDLKGFEFGVFDWH 358 W TYAA+QSL +++ S +P ++ K LKA+ TV+GPL WDEKGDLKGFEFGVF WH Sbjct: 301 WITYAAVQSLAQAMDRAASQEPLDLIKDLKAHGAKTVIGPLNWDEKGDLKGFEFGVFQWH 360 Query: 359 ANGTATDAK 367 A+G++T AK Sbjct: 361 ADGSSTLAK 369 Lambda K H 0.314 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 523 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 367 Length of database: 369 Length adjustment: 30 Effective length of query: 337 Effective length of database: 339 Effective search space: 114243 Effective search space used: 114243 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory