GapMind for catabolism of small carbon sources

 

xylitol catabolism in Klebsiella michiganensis M5al

Best path

fruI, x5p-reductase

Also see fitness data for the top candidates

Rules

Overview: Xylitol utilization in GapMind is based on the MetaCyc pathway via xylitol dehydrogenase (link) or on utilization via a phosphotransferase system and D-xylulose-5-phosphate 2-reductase (PMID:27553222).

19 steps (12 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
fruI xylitol PTS, enzyme IIABC (FruI) BWI76_RS24865 BWI76_RS19725
x5p-reductase D-xylulose-5-phosphate 2-reductase BWI76_RS11535 BWI76_RS24895
Alternative steps:
Dshi_0546 xylitol ABC transporter, ATPase component BWI76_RS26290 BWI76_RS06690
Dshi_0547 xylitol ABC transporter, substrate-binding component
Dshi_0548 xylitol ABC transporter, permease component 1
Dshi_0549 xylitol ABC transporter, permease component 2 BWI76_RS06705 BWI76_RS11245
EIIA-Axl xylitol PTS, enzyme IIA (EIIA-Axl)
EIIB-Axl xylitol PTS, enzyme IIB (EIIB-Axl)
EIIC-Axl xylitol PTS, enzyme IIC (EIIC-Axl) BWI76_RS24890 BWI76_RS14905
HSERO_RS17000 xylitol ABC transporter, substrate-binding component
HSERO_RS17005 xylitol ABC transporter, permease component 1
HSERO_RS17010 xylitol ABC transporter, permease component 2
HSERO_RS17020 xylitol ABC transporter, ATPase component BWI76_RS26290 BWI76_RS23390
PLT5 xylitol:H+ symporter PLT5 BWI76_RS03110
PS417_12055 xylitol ABC transporter, substrate-binding component BWI76_RS07235
PS417_12060 xylitol ABC transporter, permease component BWI76_RS00280 BWI76_RS14865
PS417_12065 xylitol ABC transporter, ATPase component BWI76_RS07240 BWI76_RS00275
xdhA xylitol dehydrogenase BWI76_RS11545 BWI76_RS11535
xylB xylulokinase BWI76_RS27020 BWI76_RS19340

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory