Alignments for a candidate for EIIC-Axl in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for EIIC-Axl in Klebsiella michiganensis M5al

Align PTS system, galactitol-specific, IIC component, component of Pentitol [arabitol (arabinitol, lyxitol)/xylitol] PTS permease, IICBA (encoded within an operon that also encodes a polyol-P dehydrogenase, a ribose-5-P isomerase, a ribulose 5-P-3-epimerase, and a BlgG-type PTS antiterminator) (Saklani-Jusforgues et al., 2001; Kentache T et al., 2016, in press) (characterized)
to candidate BWI76_RS24890 BWI76_RS24890 PTS system galactitol-specific transporter subunit IIC

Query= TCDB::Q71WA6
         (423 letters)



>FitnessBrowser__Koxy:BWI76_RS24890
          Length = 457

 Score =  355 bits (911), Expect = e-102
 Identities = 167/407 (41%), Positives = 271/407 (66%), Gaps = 2/407 (0%)

Query: 8   VQYVLNLGPTVILPIMIFFIALIFRVPAKKALRSAITIGIGFVGINLVISLLSSNLGPAA 67
           ++Y+L+LGPTV+LPI+I   + I  +      +S + IGIGFVGI LVI L+  ++GPAA
Sbjct: 6   MRYILDLGPTVMLPIVIIIFSKILGMKLGDCFKSGLHIGIGFVGIGLVIGLMLDSIGPAA 65

Query: 68  QQMVERFGLNLTIIDAGWPAAAAASWASPVAAILIPICLVVNLALIFFKVTKTLDIDIWN 127
           + M E F +NL +ID GWP ++  +WAS +A + IPI + VN+ ++  ++T+ +++DIWN
Sbjct: 66  KAMAEHFQINLHVIDIGWPGSSPMTWASQIALVAIPIAIAVNIFMLVTRMTRVVNVDIWN 125

Query: 128 YWHFIAAGATGYIVTGGNWWFAILCAIIYEVAVLWMADRTQPMVEEFYGLKGISLPTGST 187
            WH    GA  +I TG ++W  IL  +++   V  + D       +F+GL+GI++P GS+
Sbjct: 126 IWHMTFTGAMLHIATG-SYWIGILGVVVHAAFVYKLGDWFAKDTRDFFGLEGIAIPHGSS 184

Query: 188 AAFGFIGIPVGWLIAKIPGIKNIHVDPETIQKRFGIFGEPMMMGLILGIAIGILAGYDVG 247
           A  G I + V  +I KIPG+  IH   + IQKRFG FGEP+ +G ++G+ IG LAGYD+ 
Sbjct: 185 AYLGPIAVLVDTIIEKIPGLNRIHFSADDIQKRFGPFGEPVTVGFVMGLVIGALAGYDLK 244

Query: 248 AVAQLGMSMGAVMFLMPRMVKILMEGLIPISESAREFMKSRFKGRELYIGLDAALSIGHP 307
            + QL +   AVM LMPR++K +M+GL PI++ AR+ ++++F G++  IGLD AL +GH 
Sbjct: 245 GILQLAVKTAAVMLLMPRVIKPIMDGLNPIAKQARKRLQAKFGGQDFLIGLDPALLLGHT 304

Query: 308 ANISTGLILVPITLFLAVIIPGNKVLPFGDLATIPFYVSFVVASRKGNILHSVLAGTVVI 367
           + +S  LI +P+++ +AV++PGN+VLPFGDLATI F+V+  VA  +GN+  ++++G +++
Sbjct: 305 SVVSASLIFIPLSILIAVLVPGNQVLPFGDLATIGFFVAMAVAVHQGNLFRTLISGIILM 364

Query: 368 ALALLMATDFGLVHTEMMKGVYEFPKGATQVSTLDMGGNFFNWVILK 414
            + L +AT    +HT++         G   V+++D GG+   W++++
Sbjct: 365 GITLWIATQTIGLHTQLAANAGALKAGGL-VASMDQGGSPITWLLIQ 410



 Score = 31.2 bits (69), Expect = 7e-05
 Identities = 43/192 (22%), Positives = 74/192 (38%), Gaps = 9/192 (4%)

Query: 25  FFIALIFRVPAKKALRSAITIGIGFVGINL----VISLLSSNLGPAAQQMVERFGLNLTI 80
           F + L+    A   L+  + + +    + L    VI  +   L P A+Q  +R       
Sbjct: 229 FVMGLVIGALAGYDLKGILQLAVKTAAVMLLMPRVIKPIMDGLNPIAKQARKRLQAKFGG 288

Query: 81  ID--AGWPAAAAASWASPVAAILIPICLVVNLALIFFKVTKTLDIDIWNYWHFIAAGATG 138
            D   G   A      S V+A LI I L + +A++          D+     F+A     
Sbjct: 289 QDFLIGLDPALLLGHTSVVSASLIFIPLSILIAVLVPGNQVLPFGDLATIGFFVAMAVA- 347

Query: 139 YIVTGGNWWFAILCAIIYEVAVLWMADRTQPMVEEFYGLKGISLPTGSTAAFGFIGIPVG 198
             V  GN +  ++  II     LW+A +T  +  +     G     G  A+    G P+ 
Sbjct: 348 --VHQGNLFRTLISGIILMGITLWIATQTIGLHTQLAANAGALKAGGLVASMDQGGSPIT 405

Query: 199 WLIAKIPGIKNI 210
           WL+ ++   +N+
Sbjct: 406 WLLIQLFTWQNV 417


Lambda     K      H
   0.328    0.143    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 554
Number of extensions: 33
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 423
Length of database: 457
Length adjustment: 32
Effective length of query: 391
Effective length of database: 425
Effective search space:   166175
Effective search space used:   166175
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources