Align Xylose isomerase (EC 5.3.1.5) (characterized)
to candidate BWI76_RS27025 BWI76_RS27025 xylose isomerase
Query= reanno::Koxy:BWI76_RS27025 (440 letters) >FitnessBrowser__Koxy:BWI76_RS27025 Length = 440 Score = 915 bits (2366), Expect = 0.0 Identities = 440/440 (100%), Positives = 440/440 (100%) Query: 1 MQTYFDQLDRVRYEGPKSANPLAFRHYNPDELVLGKRMEDHLRFAACYWHTFCWNGADMF 60 MQTYFDQLDRVRYEGPKSANPLAFRHYNPDELVLGKRMEDHLRFAACYWHTFCWNGADMF Sbjct: 1 MQTYFDQLDRVRYEGPKSANPLAFRHYNPDELVLGKRMEDHLRFAACYWHTFCWNGADMF 60 Query: 61 GVGSFNRPWQQPGEAMEMAKRKADVAFEFFHKLNVPYYCFHDVDVSPEGASLKEYANNFA 120 GVGSFNRPWQQPGEAMEMAKRKADVAFEFFHKLNVPYYCFHDVDVSPEGASLKEYANNFA Sbjct: 61 GVGSFNRPWQQPGEAMEMAKRKADVAFEFFHKLNVPYYCFHDVDVSPEGASLKEYANNFA 120 Query: 121 QMVDVLAEKQQQSGVKLLWGTANCFTNPRYGAGAATNPDPEVFSWAATQVVTAMDATHKL 180 QMVDVLAEKQQQSGVKLLWGTANCFTNPRYGAGAATNPDPEVFSWAATQVVTAMDATHKL Sbjct: 121 QMVDVLAEKQQQSGVKLLWGTANCFTNPRYGAGAATNPDPEVFSWAATQVVTAMDATHKL 180 Query: 181 GGENYVLWGGREGYETLLNTDLRQEREQIGRFMQLVVEHKHKIGFQGTLLIEPKPQEPTK 240 GGENYVLWGGREGYETLLNTDLRQEREQIGRFMQLVVEHKHKIGFQGTLLIEPKPQEPTK Sbjct: 181 GGENYVLWGGREGYETLLNTDLRQEREQIGRFMQLVVEHKHKIGFQGTLLIEPKPQEPTK 240 Query: 241 HQYDYDASTVYGFLKQFGLEKEIKLNIEANHATLAGHTFHHEIATAIALGLFGSVDANRG 300 HQYDYDASTVYGFLKQFGLEKEIKLNIEANHATLAGHTFHHEIATAIALGLFGSVDANRG Sbjct: 241 HQYDYDASTVYGFLKQFGLEKEIKLNIEANHATLAGHTFHHEIATAIALGLFGSVDANRG 300 Query: 301 DPQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAM 360 DPQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAM Sbjct: 301 DPQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAM 360 Query: 361 DTMALALKVAARMIEGGELDKRVAKRYAGWNGELGQQILKGQMNLADIAQYATQHNLAPQ 420 DTMALALKVAARMIEGGELDKRVAKRYAGWNGELGQQILKGQMNLADIAQYATQHNLAPQ Sbjct: 361 DTMALALKVAARMIEGGELDKRVAKRYAGWNGELGQQILKGQMNLADIAQYATQHNLAPQ 420 Query: 421 HQSGHQELLENVVNRYLFDR 440 HQSGHQELLENVVNRYLFDR Sbjct: 421 HQSGHQELLENVVNRYLFDR 440 Lambda K H 0.320 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 904 Number of extensions: 21 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 440 Length of database: 440 Length adjustment: 32 Effective length of query: 408 Effective length of database: 408 Effective search space: 166464 Effective search space used: 166464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate BWI76_RS27025 BWI76_RS27025 (xylose isomerase)
to HMM TIGR02630 (xylA: xylose isomerase (EC 5.3.1.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02630.hmm # target sequence database: /tmp/gapView.5677.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02630 [M=435] Accession: TIGR02630 Description: xylose_isom_A: xylose isomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-223 728.4 1.1 1.7e-223 728.2 1.1 1.0 1 lcl|FitnessBrowser__Koxy:BWI76_RS27025 BWI76_RS27025 xylose isomerase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Koxy:BWI76_RS27025 BWI76_RS27025 xylose isomerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 728.2 1.1 1.7e-223 1.7e-223 1 435 [] 4 437 .. 4 437 .. 1.00 Alignments for each domain: == domain 1 score: 728.2 bits; conditional E-value: 1.7e-223 TIGR02630 1 ffeeiekvkyeGkdsknplafkyynpeeviegkkmkdhlrfavayWhtlaaegaDpfGagtaerpwdkelt 71 +f+++++v+yeG++s nplaf++ynp+e++ gk+m+dhlrfa +yWht++++gaD+fG g+++rpw++ lcl|FitnessBrowser__Koxy:BWI76_RS27025 4 YFDQLDRVRYEGPKSANPLAFRHYNPDELVLGKRMEDHLRFAACYWHTFCWNGADMFGVGSFNRPWQQP-G 73 8*****************************************************************965.9 PP TIGR02630 72 daldkakakvdaafelleklgveyycfhDvDiapegaslketnanldeivdllkekqketgvklLWgtanl 142 +a+++ak+k+d+afe+++kl+v+yycfhDvD++pegaslke+ +n+ ++vd+l ekq+++gvklLWgtan+ lcl|FitnessBrowser__Koxy:BWI76_RS27025 74 EAMEMAKRKADVAFEFFHKLNVPYYCFHDVDVSPEGASLKEYANNFAQMVDVLAEKQQQSGVKLLWGTANC 144 *********************************************************************** PP TIGR02630 143 fshpryvaGaatspdadvfayaaaqvkkalevtkklggenyvfWGGreGyetLlntdlkleldnlarflkl 213 f++pry aGaat+pd++vf++aa+qv +a+++t+klggenyv+WGGreGyetLlntdl++e+++++rf++l lcl|FitnessBrowser__Koxy:BWI76_RS27025 145 FTNPRYGAGAATNPDPEVFSWAATQVVTAMDATHKLGGENYVLWGGREGYETLLNTDLRQEREQIGRFMQL 215 *********************************************************************** PP TIGR02630 214 avdyakkigfkgqlliePkPkePtkhqyDfDaatvlaflkkydLdkdfklnieanhatLaghtfehelrva 284 +v++++kigf+g+lliePkP+ePtkhqyD+Da+tv++flk+++L+k++klnieanhatLaghtf+he+++a lcl|FitnessBrowser__Koxy:BWI76_RS27025 216 VVEHKHKIGFQGTLLIEPKPQEPTKHQYDYDASTVYGFLKQFGLEKEIKLNIEANHATLAGHTFHHEIATA 286 *********************************************************************** PP TIGR02630 285 relgllGsiDanrgdlllGWDtDefptdvyeatlamyevlkagglakGGlnfdakvrresldaeDlllahi 355 +lgl+Gs+Danrgd +lGWDtD+fp++v+e++l+mye+lkagg+++GGlnfdakvrr+s+d+ Dl+++hi lcl|FitnessBrowser__Koxy:BWI76_RS27025 287 IALGLFGSVDANRGDPQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHI 357 *********************************************************************** PP TIGR02630 356 agmDafarglkvaaklledgaleklveeryesfdseiGkeieegkadleelekyalekeeeaekksgrqel 426 ++mD++a++lkvaa+++e g+l+k v++ry++++ e+G++i++g+++l+++++ya++++ +++++sg+qel lcl|FitnessBrowser__Koxy:BWI76_RS27025 358 GAMDTMALALKVAARMIEGGELDKRVAKRYAGWNGELGQQILKGQMNLADIAQYATQHNLAPQHQSGHQEL 428 *********************************************************************** PP TIGR02630 427 leslinkyl 435 le+++n+yl lcl|FitnessBrowser__Koxy:BWI76_RS27025 429 LENVVNRYL 437 *******96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (435 nodes) Target sequences: 1 (440 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.60 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory