GapMind for catabolism of small carbon sources

 

Aligments for a candidate for xylT in Klebsiella michiganensis M5al

Align D-xylose-proton symporter (characterized)
to candidate BWI76_RS10225 BWI76_RS10225 MFS transporter

Query= CharProtDB::CH_109760
         (491 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS10225 BWI76_RS10225 MFS
           transporter
          Length = 483

 Score =  310 bits (795), Expect = 5e-89
 Identities = 167/490 (34%), Positives = 283/490 (57%), Gaps = 41/490 (8%)

Query: 6   NSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASAL 65
           N  Y+  I  +A++ G++ GYD +VISG ++ L       ++LS + A S  G+ V++ +
Sbjct: 7   NVPYLIFICAIASVSGIILGYDASVISGVIDPLT------EHLSLTPAQS--GWAVSNVI 58

Query: 66  IGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGY 125
           +GCI+G    G  ++RFGR+ +L I A+LF IS +GSA                  LA  
Sbjct: 59  LGCIVGAWGVGRFTDRFGRKATLIITAILFAISAIGSA------------------LAND 100

Query: 126 VPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFI 185
           +  FV+YR+IGG+ VG+AS ++P+YIAE++P  +RG+++   Q  ++ GQL+VY VNY I
Sbjct: 101 LTWFVVYRMIGGLAVGMASAVTPLYIAEVSPKDLRGRMLGMQQMLMVGGQLVVYIVNYLI 160

Query: 186 ARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMG 245
           AR     W+ + GWR+M AS  IP +LFL++++ +PESPRW   R ++E++  +L  +  
Sbjct: 161 ARGMAHEWVVSIGWRWMLASALIPCVLFLVMVFFMPESPRWYAMRNQKEKSLKVLTSLSN 220

Query: 246 NTLATQAVQEIKHSLDHGRKTGGRLLMFGVG-----------VIVIGVMLSIFQQFVGIN 294
              A +   EI+ S+     T   LL                ++ IG  +++ QQ  GIN
Sbjct: 221 PGHAERLYAEIRTSI----ATDSALLSIPTPRGILRDKKSSYILWIGCAIAVLQQVSGIN 276

Query: 295 VVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAI 354
           +++Y+AP + + +  +T  ++ Q+I +G+  L    +A++  D+ GR PL   G+LG A 
Sbjct: 277 ILMYFAPSLLQNVTGNTQDSMFQSIFLGLALLAGVSIALVAFDRIGRLPLLRWGSLGCAA 336

Query: 355 GMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAA 414
            +     AF  +  G + ++ ++ ++  F MSW    W+L+SEIFPN +R  A+  A  +
Sbjct: 337 FLLFTSWAFMNEVKGYLPIVGLVGFIFVFGMSWSLGAWLLISEIFPNRMRAVAMGYAFCS 396

Query: 415 QWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKGKTLEEL 474
            W++N+ V+ +FPMM++N  L+ HFH  F   +     ++A  F+ +F+PETKG +LE +
Sbjct: 397 MWVSNFIVTQSFPMMNRNPVLMEHFHGAFPLLLCSGFSIIAFWFVGRFLPETKGVSLENI 456

Query: 475 EALWEPETKK 484
           E L   ++++
Sbjct: 457 EPLMLSKSRR 466


Lambda     K      H
   0.326    0.140    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 636
Number of extensions: 35
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 491
Length of database: 483
Length adjustment: 34
Effective length of query: 457
Effective length of database: 449
Effective search space:   205193
Effective search space used:   205193
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory