Alignments for a candidate for xylT in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for xylT in Klebsiella michiganensis M5al

Align Arabinose-proton symporter; Arabinose transporter (characterized)
to candidate BWI76_RS24055 BWI76_RS24055 MFS transporter

Query= SwissProt::P0AE24
         (472 letters)



>FitnessBrowser__Koxy:BWI76_RS24055
          Length = 464

 Score =  594 bits (1532), Expect = e-174
 Identities = 298/456 (65%), Positives = 366/456 (80%), Gaps = 3/456 (0%)

Query: 16  RDTRRMNMFVSVAAAVAGLLFGLDIGVIAGALPFITDHFVLTSRLQEWVVSSMMLGAAIG 75
           R  + M  FV   AA+AGLLFGLDIGVIAGALPFI + F +++  QEWVVSSMM GAA+G
Sbjct: 9   RSNKTMTFFVCFLAALAGLLFGLDIGVIAGALPFIANEFQISAHTQEWVVSSMMFGAAVG 68

Query: 76  ALFNGWLSFRLGRKYSLMAGAILFVLGSIGSAFATSVEMLIAARVVLGIAVGIASYTAPL 135
           A+ +GWLSF+LGRK SLM GAILFV GS+ SA A +VE+L+ +RV+LG+AVG+ASYTAPL
Sbjct: 69  AVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAAPNVEVLLISRVLLGLAVGVASYTAPL 128

Query: 136 YLSEMASENVRGKMISMYQLMVTLGIVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILV 195
           YLSE+A E +RG MISMYQLM+T+GI+ A+LSDTAFSYSG WR MLGV+ +PAVLL+I V
Sbjct: 129 YLSEIAPEKIRGSMISMYQLMITIGILGAYLSDTAFSYSGAWRWMLGVIIIPAVLLLIGV 188

Query: 196 VFLPNSPRWLAEKGRHIEAEEVLRMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNV 255
           VFLP+SPRW A K R ++AE VL  LRDTS +A+ EL+EIRESLK+KQ GW+LFK N N 
Sbjct: 189 VFLPDSPRWFAAKRRFVDAERVLLRLRDTSAEAKRELDEIRESLKVKQSGWSLFKENSNF 248

Query: 256 RRAVFLGMLLQAMQQFTGMNIIMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAV 315
           RRAVFLG+LLQ MQQFTGMN+IMYYAP+IF++AG+  T +QM  T++VGLT + ATFIA+
Sbjct: 249 RRAVFLGVLLQVMQQFTGMNVIMYYAPKIFELAGYANTTEQMWGTVIVGLTNVLATFIAI 308

Query: 316 FTVDKAGRKPALKIGFSVMALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMS 375
             VD+ GRKP L +GF VMALG  +LG        G  S+   + +V M +M I G+AMS
Sbjct: 309 GLVDRWGRKPTLILGFIVMALGMGILG---SMMHIGIHSATAQYFAVLMLLMFIVGFAMS 365

Query: 376 AAPVVWILCSEIQPLKCRDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALN 435
           A P++W+LCSEIQPLK RDFGITCST TNW++NMI+GATFLT+L+S+G+A TFW+Y  LN
Sbjct: 366 AGPLIWVLCSEIQPLKGRDFGITCSTATNWIANMIVGATFLTMLNSLGSANTFWVYGGLN 425

Query: 436 IAFVGITFWLIPETKNVTLEHIERKLMAGEKLRNIG 471
           I F+ +T WLIPETKNV+LEHIER LM G KLR IG
Sbjct: 426 ILFIFLTLWLIPETKNVSLEHIERNLMKGRKLREIG 461


Lambda     K      H
   0.327    0.138    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 768
Number of extensions: 40
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 472
Length of database: 464
Length adjustment: 33
Effective length of query: 439
Effective length of database: 431
Effective search space:   189209
Effective search space used:   189209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources