GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gabD in Shewanella oneidensis MR-1

Align Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized)
to candidate 200843 SO1678 methylmalonate-semialdehyde dehydrogenase (NCBI ptt file)

Query= SwissProt::Q8GAK7
         (458 letters)



>lcl|FitnessBrowser__MR1:200843 SO1678 methylmalonate-semialdehyde
           dehydrogenase (NCBI ptt file)
          Length = 499

 Score =  181 bits (459), Expect = 5e-50
 Identities = 140/468 (29%), Positives = 219/468 (46%), Gaps = 27/468 (5%)

Query: 3   IATIDPTTGITLKTFDAHTPEEVENRIARAEAAFRSLQNTSFEERARWMHKAADILESEA 62
           I   +P    T+   ++ T +EV   IA A+AAF++ +     ERAR M +   +L+   
Sbjct: 22  IVVTNPANNATIAVINSATADEVHAAIASAKAAFKTWKEVPVSERARVMLRYQHLLKEHH 81

Query: 63  DEVARLIATEMGKTLTTAKYEALKSATGMRHFADHAQRYLSPETPVPASEVNASNLHVQF 122
           DE+A ++A E GKT   AK +  +      H  + A   +       A  ++    +   
Sbjct: 82  DELATILAHETGKTFEDAKGDVWRGIEVAEHACNIASLLMGETVENVARSIDT---YSYT 138

Query: 123 DPLGVVLAVMPWNYPLWQAVRFAAPALMAGNTGLLKHASNVPQCALYLGDLFARGGFPEG 182
            PLGV   + P+N+P    +     A+  GNT +LK +   P     L +LF   G P+G
Sbjct: 139 QPLGVCAGITPFNFPAMIPLWMFPLAIACGNTFILKPSEQDPMTPQRLVELFVEAGAPKG 198

Query: 183 AFQTLLVEGKDVIPLVDDARIRAVTLTGSVAAGSAIAEAAGRNIKRSVLELGGMDVFIVM 242
             Q +  +   V  L+ D  ++A++  GSVA G  I +    N+KR     G  +  ++M
Sbjct: 199 VLQLIHGDKTAVDILLADPAVKAISFVGSVAVGQYIYKTGTDNLKRVQAFAGAKNHCVIM 258

Query: 243 PSADIEKAAAQAVIARLQNSGQSCIAAKRFYVHEDVYDRFEHLFVTGMAEAVA----GDP 298
           P A+ ++     V A +  +GQ C+A     +   V+      ++  + EA+A    G  
Sbjct: 259 PDANKQQVINNLVGASVGAAGQRCMA-----ISVAVFVGAAKEWIPELKEALAKVRPGLW 313

Query: 299 LDESTSFGPLATERGRQDVHELVRDAREKGAAVQC---GGEIP----EGEGWYYPATVLT 351
            D+   +GPL +   +  V +L+   +E+GA  QC   G +      E   W  P T+ T
Sbjct: 314 DDKDAGYGPLISPAAKVRVLKLIAQGKEEGA--QCLLDGSDFTVAGFESGNWVGP-TMFT 370

Query: 352 GVTEDMRIYREECFGPVACLYKVSSLQEAIALSNDSDFGLSSSVWTNDETEATEAARSIE 411
            VT DM IY+EE FGPV C  +  SL++AI L N S +G  +S++T     A +    IE
Sbjct: 371 KVTTDMSIYKEEIFGPVLCCMESDSLEDAIELVNASPYGNGTSIFTASGAAARKYQHEIE 430

Query: 412 AGGVFIN-GLTASFPAVPFGGLKDSGYGRELSAYG---IREFVNIKTV 455
            G V IN  +    P   F G K S YG +  AYG   +R +   KT+
Sbjct: 431 VGQVGINVPIPVPLPFFSFTGWKGSFYG-DQHAYGKQAVRFYTETKTI 477


Lambda     K      H
   0.317    0.132    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 491
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 458
Length of database: 499
Length adjustment: 34
Effective length of query: 424
Effective length of database: 465
Effective search space:   197160
Effective search space used:   197160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory